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Angew Chem Int Ed Engl
2021 Jun 01;6023:12852-12858. doi: 10.1002/anie.202100923.
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In Vivo Subcellular Mass Spectrometry Enables Proteo-Metabolomic Single-Cell Systems Biology in a Chordate Embryo Developing to a Normally Behaving Tadpole (X. laevis)*.
Lombard-Banek C
,
Li J
,
Portero EP
,
Onjiko RM
,
Singer CD
,
Plotnick DO
,
Al Shabeeb RQ
,
Nemes P
.
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We report the development of in vivo subcellular high-resolution mass spectrometry (HRMS) for proteo-metabolomic molecular systems biology in complex tissues. With light microscopy, we identified the left-dorsal and left-ventral animal cells in cleavage-stage non-sentient Xenopus laevis embryos. Using precision-translated fabricated microcapillaries, the subcellular content of each cell was double-probed, each time swiftly (<5 s/event) aspirating <5 % of cell volume (≈10 nL). The proteins and metabolites were analyzed by home-built ultrasensitive capillary electrophoresis electrospray ionization employing orbitrap or time-of-flight HRMS. Label-free detection of ≈150 metabolites (57 identified) and 738 proteins found proteo-metabolomic networks with differential quantitative activities between the cell types. With spatially and temporally scalable sampling, the technology preserved the integrity of the analyzed cells, the neighboring cells, and the embryo. 95 % of the analyzed embryos developed into sentient tadpoles that were indistinguishable from their wild-type siblings based on anatomy and visual function in a background color preference assay.
Briggs,
The dynamics of gene expression in vertebrate embryogenesis at single-cell resolution.
2018, Pubmed,
Xenbase
Briggs,
The dynamics of gene expression in vertebrate embryogenesis at single-cell resolution.
2018,
Pubmed
,
Xenbase
Budnik,
SCoPE-MS: mass spectrometry of single mammalian cells quantifies proteome heterogeneity during cell differentiation.
2018,
Pubmed
Comi,
Categorizing Cells on the Basis of their Chemical Profiles: Progress in Single-Cell Mass Spectrometry.
2017,
Pubmed
Cong,
Improved Single-Cell Proteome Coverage Using Narrow-Bore Packed NanoLC Columns and Ultrasensitive Mass Spectrometry.
2020,
Pubmed
Couvillion,
New mass spectrometry technologies contributing towards comprehensive and high throughput omics analyses of single cells.
2019,
Pubmed
De Domenico,
Molecular asymmetry in the 8-cell stage Xenopus tropicalis embryo described by single blastomere transcript sequencing.
2015,
Pubmed
,
Xenbase
Do,
Optically Guided Single Cell Mass Spectrometry of Rat Dorsal Root Ganglia to Profile Lipids, Peptides and Proteins.
2018,
Pubmed
Flachsova,
Single blastomere expression profiling of Xenopus laevis embryos of 8 to 32-cells reveals developmental asymmetry.
2013,
Pubmed
,
Xenbase
Hirose,
Clonal organization of the central nervous system of the frog. I. Clones stemming from individual blastomeres of the 16-cell and earlier stages.
1979,
Pubmed
,
Xenbase
Kanehisa,
KEGG: new perspectives on genomes, pathways, diseases and drugs.
2017,
Pubmed
Karimi,
Xenbase: a genomic, epigenomic and transcriptomic model organism database.
2018,
Pubmed
,
Xenbase
Kelly,
Single-cell Proteomics: Progress and Prospects.
2020,
Pubmed
Lapainis,
Capillary electrophoresis with electrospray ionization mass spectrometric detection for single-cell metabolomics.
2009,
Pubmed
Li,
An Integrated Platform for Isolation, Processing, and Mass Spectrometry-based Proteomic Profiling of Rare Cells in Whole Blood.
2015,
Pubmed
Liu,
Analysis of endogenous nucleotides by single cell capillary electrophoresis-mass spectrometry.
2014,
Pubmed
Lombard-Banek,
Label-free Quantification of Proteins in Single Embryonic Cells with Neural Fate in the Cleavage-Stage Frog (Xenopus laevis) Embryo using Capillary Electrophoresis Electrospray Ionization High-Resolution Mass Spectrometry (CE-ESI-HRMS).
2016,
Pubmed
,
Xenbase
Lombard-Banek,
Microsampling Capillary Electrophoresis Mass Spectrometry Enables Single-Cell Proteomics in Complex Tissues: Developing Cell Clones in Live Xenopus laevis and Zebrafish Embryos.
2019,
Pubmed
,
Xenbase
Ma,
Integrative Methods and Practical Challenges for Single-Cell Multi-omics.
2020,
Pubmed
Macaulay,
Single-Cell Multiomics: Multiple Measurements from Single Cells.
2017,
Pubmed
Nemes,
Qualitative and quantitative metabolomic investigation of single neurons by capillary electrophoresis electrospray ionization mass spectrometry.
2013,
Pubmed
,
Xenbase
Nemes,
Metabolic differentiation of neuronal phenotypes by single-cell capillary electrophoresis-electrospray ionization-mass spectrometry.
2011,
Pubmed
Onjiko,
In Situ Microprobe Single-Cell Capillary Electrophoresis Mass Spectrometry: Metabolic Reorganization in Single Differentiating Cells in the Live Vertebrate (Xenopus laevis) Embryo.
2017,
Pubmed
,
Xenbase
Onjiko,
Metabolic Comparison of Dorsal versus Ventral Cells Directly in the Live 8-cell Frog Embryo by Microprobe Single-cell CE-ESI-MS.
2017,
Pubmed
,
Xenbase
Onjiko,
Single-cell mass spectrometry reveals small molecules that affect cell fates in the 16-cell embryo.
2015,
Pubmed
,
Xenbase
Onjiko,
Microprobe Capillary Electrophoresis Mass Spectrometry for Single-cell Metabolomics in Live Frog (Xenopus laevis) Embryos.
2017,
Pubmed
,
Xenbase
Portero,
Dual cationic-anionic profiling of metabolites in a single identified cell in a live Xenopus laevis embryo by microprobe CE-ESI-MS.
2019,
Pubmed
,
Xenbase
Schrimpe-Rutledge,
Untargeted Metabolomics Strategies-Challenges and Emerging Directions.
2016,
Pubmed
Specht,
Single-cell proteomic and transcriptomic analysis of macrophage heterogeneity using SCoPE2.
2021,
Pubmed
Specht,
Transformative Opportunities for Single-Cell Proteomics.
2018,
Pubmed
Sun,
Single Cell Proteomics Using Frog (Xenopus laevis) Blastomeres Isolated from Early Stage Embryos, Which Form a Geometric Progression in Protein Content.
2016,
Pubmed
,
Xenbase
Viczian,
A simple behavioral assay for testing visual function in Xenopus laevis.
2014,
Pubmed
,
Xenbase
Virant-Klun,
Identification of Maturation-Specific Proteins by Single-Cell Proteomics of Human Oocytes.
2016,
Pubmed
Zenobi,
Single-cell metabolomics: analytical and biological perspectives.
2013,
Pubmed
Zhang,
Single-Cell Mass Spectrometry Approaches to Explore Cellular Heterogeneity.
2018,
Pubmed
Zhu,
Proteomic Analysis of Single Mammalian Cells Enabled by Microfluidic Nanodroplet Sample Preparation and Ultrasensitive NanoLC-MS.
2018,
Pubmed
