Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
XB-ART-58006
Tissue Cell 2021 Oct 01;72:101531. doi: 10.1016/j.tice.2021.101531.
Show Gene links Show Anatomy links

Pou5f3.3 is involved in establishment and maintenance of hematopoietic cells during Xenopus development.

Ezawa M , Kouno F , Kubo H , Sakuma T , Yamamoto T , Kinoshita T .


???displayArticle.abstract???
Three POU family class V gene homologues are expressed in the development of Xenopus. In contrast to the expression of Pou5f3.1 and Pou5f3.2 in organogenesis, Pou5f3.3 is expressed during oogenesis in ovary. We investigated the expression and function of Pou5f3.3 in organogenesis of Xenopus laevis. RT-PCR and immunohistochemical analysis indicated that Pou5f3.3 was expressed in a small number of adult liver cells and blood cells. Immunocytochemical investigation proved that Bmi1, a marker for hematopoietic progenitor cells, was co-expressed in Pou5f3.3-expressing small spherical cells in the peripheral blood. In anemic induction by intraperitoneal injection of phenyl hydrazine, the number of Pou5f3.3-expressing cells significantly increased within 3 days after phenyl hydrazine injection. In CRISPR/Cas mutagenesis of Pou5f3.3, Bmi1-positive hematopoietic progenitor cell count decreased in the hematopoietic dorsal-lateral plate (DLP) region, resulting in a considerable reduction in peripheral blood cells. CRISPR/Cas-induced hematopoietic deficiency was completely rescued by Pou5f3.3 supplementation, but not by Pou5f3.1 or Pou5f3.2. Transplantation experiments using the H2B-GFP transgenic line demonstrated that DLP-derived Pou5f3.3-positive and Bmi1-positive cells were translocated into the liver and bone through the bloodstream. These results suggest that Pou5f3.3 plays an essential role in the establishment and maintenance of hematopoietic progenitor cells during Xenopus development.

???displayArticle.pubmedLink??? 33798831
???displayArticle.link??? Tissue Cell


Species referenced: Xenopus laevis
Genes referenced: bmi1 h2bc21 mpo pou5f3 pou5f3.2 pou5f3.3 runx1 tal1 tuba1c
GO keywords: hematopoietic stem cell differentiation
???displayArticle.antibodies??? Bmi1 AB1 Pou5f3.3 AB1
gRNAs referenced: pou5f3.3 gRNA1 pou5f3.3 gRNA2