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Extending the Anion Channelrhodopsin-Based Toolbox for Plant Optogenetics.
Zhou Y
,
Ding M
,
Duan X
,
Konrad KR
,
Nagel G
,
Gao S
.
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Optogenetics was developed in the field of neuroscience and is most commonly using light-sensitive rhodopsins to control the neural activities. Lately, we have expanded this technique into plant science by co-expression of a chloroplast-targeted β-carotene dioxygenase and an improved anion channelrhodopsin GtACR1 from the green alga Guillardia theta. The growth of Nicotiana tabacum pollen tube can then be manipulated by localized green light illumination. To extend the application of analogous optogenetic tools in the pollen tube system, we engineered another two ACRs, GtACR2, and ZipACR, which have different action spectra, light sensitivity and kinetic features, and characterized them in Xenopus laevis oocytes, Nicotiana benthamiana leaves and N. tabacum pollen tubes. We found that the similar molecular engineering method used to improve GtACR1 also enhanced GtACR2 and ZipACR performance in Xenopus laevis oocytes. The ZipACR1 performed in N. benthamiana mesophyll cells and N. tabacum pollen tubes with faster kinetics and reduced light sensitivity, allowing for optogenetic control of anion fluxes with better temporal resolution. The reduced light sensitivity would potentially facilitate future application in plants, grown under low ambient white light, combined with an optogenetic manipulation triggered by stronger green light.
Adamantidis,
Optogenetics: 10 years after ChR2 in neurons--views from the community.
2015, Pubmed
Adamantidis,
Optogenetics: 10 years after ChR2 in neurons--views from the community.
2015,
Pubmed
Bi,
Ectopic expression of a microbial-type rhodopsin restores visual responses in mice with photoreceptor degeneration.
2006,
Pubmed
Boyden,
Millisecond-timescale, genetically targeted optical control of neural activity.
2005,
Pubmed
Chatelle,
A Green-Light-Responsive System for the Control of Transgene Expression in Mammalian and Plant Cells.
2018,
Pubmed
Dawydow,
Channelrhodopsin-2-XXL, a powerful optogenetic tool for low-light applications.
2014,
Pubmed
Govorunova,
NEUROSCIENCE. Natural light-gated anion channels: A family of microbial rhodopsins for advanced optogenetics.
2015,
Pubmed
Govorunova,
The Expanding Family of Natural Anion Channelrhodopsins Reveals Large Variations in Kinetics, Conductance, and Spectral Sensitivity.
2017,
Pubmed
Gradinaru,
Molecular and cellular approaches for diversifying and extending optogenetics.
2010,
Pubmed
Guru,
Making Sense of Optogenetics.
2015,
Pubmed
Gutermuth,
Tip-localized Ca2+ -permeable channels control pollen tube growth via kinase-dependent R- and S-type anion channel regulation.
2018,
Pubmed
,
Xenbase
Gutermuth,
Pollen tube growth regulation by free anions depends on the interaction between the anion channel SLAH3 and calcium-dependent protein kinases CPK2 and CPK20.
2013,
Pubmed
,
Xenbase
Hegemann,
From channelrhodopsins to optogenetics.
2013,
Pubmed
,
Xenbase
Li,
Fast noninvasive activation and inhibition of neural and network activity by vertebrate rhodopsin and green algae channelrhodopsin.
2005,
Pubmed
Mahn,
High-efficiency optogenetic silencing with soma-targeted anion-conducting channelrhodopsins.
2018,
Pubmed
Main,
Electroporation protocols for Agrobacterium.
1995,
Pubmed
Mauss,
Optogenetic Neuronal Silencing in Drosophila during Visual Processing.
2017,
Pubmed
Mohamed,
Optical inhibition of larval zebrafish behaviour with anion channelrhodopsins.
2017,
Pubmed
Mohammad,
Optogenetic inhibition of behavior with anion channelrhodopsins.
2017,
Pubmed
Mousavi,
Measuring surface potential changes on leaves.
2014,
Pubmed
Nagel,
Channelrhodopsin-2, a directly light-gated cation-selective membrane channel.
2003,
Pubmed
,
Xenbase
Nagel,
Channelrhodopsins: directly light-gated cation channels.
2005,
Pubmed
,
Xenbase
Nagel,
Light activation of channelrhodopsin-2 in excitable cells of Caenorhabditis elegans triggers rapid behavioral responses.
2005,
Pubmed
Ochoa-Fernandez,
Optogenetics in Plants: Red/Far-Red Light Control of Gene Expression.
2016,
Pubmed
Ochoa-Fernandez,
Optogenetic control of gene expression in plants in the presence of ambient white light.
2020,
Pubmed
Papanatsiou,
Optogenetic manipulation of stomatal kinetics improves carbon assimilation, water use, and growth.
2019,
Pubmed
Reyer,
Channelrhodopsin-mediated optogenetics highlights a central role of depolarization-dependent plant proton pumps.
2020,
Pubmed
,
Xenbase
Shepard,
A cleavable N-terminal signal peptide promotes widespread olfactory receptor surface expression in HEK293T cells.
2013,
Pubmed
Spudich,
Retinylidene proteins: structures and functions from archaea to humans.
2000,
Pubmed
Szymczak,
Development of 2A peptide-based strategies in the design of multicistronic vectors.
2005,
Pubmed
Terakita,
The opsins.
2005,
Pubmed
Urquiza-Garcia,
Biofortifying green optogenetics.
2021,
Pubmed
Zhou,
Optogenetic control of plant growth by a microbial rhodopsin.
2021,
Pubmed
