Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
Methods December 1, 2002; 28 (4): 402-10.

Transgenic approaches to retinal development and function in Xenopus laevis.

The African clawed frog Xenopus laevis has long been used to study the development and function of the vertebrate retina. An efficient technique for generating transgenic Xenopus embryos, the REMI procedure, has enabled the stable overexpression of transgenes in developing and mature X. laevis. In the retina, transgenes driven by retinal-specific promoters have been used to study protein trafficking, circadian rhythms, and retinal degeneration. The REMI technique is surprisingly simple, consisting of integration of plasmid DNA into permeabilized sperm nuclei, followed by transplantation of these nuclei into unfertilized eggs. Here, we describe the reagents and steps necessary for generation of transgenic embryos using the REMI reaction and discuss its applications for the study of retinal development.

PubMed ID: 12507458
Article link: Methods
Grant support: [+]

Xenbase: The Xenopus Model Organism Knowledgebase.
Version: 4.14.0
Major funding for Xenbase is provided by grant P41 HD064556