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Biochem Biophys Res Commun November 15, 2002; 298 (5): 815-23.

Transcriptional regulation of Xbr-1a/Xvent-2 homeobox gene: analysis of its promoter region.

Lee HS , Park MJ , Lee SY , Hwang YS , Lee H , Roh DH , Kim JI , Park JB , Lee JY , Kung HF , Kim J .

Xvent homeobox proteins are induced by BMP-4 signaling and have been known to mediate many BMP-4 activities as key downstream transcriptional factors. In order to investigate the regulatory mode of Xvent transcription, we isolated genomic DNA of the Xbr-1a/Xvent-2 containing the promoter region responsive to BMP-4 signaling. The cis-acting elements located within the Xbr-1a/Xvent-2 promoter and the regulation modes by BMP-4 signaling were analyzed by serial deletion and site-directed mutagenesis experiments. The upstream -235bp of the promoter retained the full transcriptional activity and BMP-4-response when compared with the longest promoter construct. Further analysis indicated that two separated 15bp regions contained a strong positive element and BMP-4-response element. Site-directed mutagenesis of those regions suggests that those two regions cooperate for the promoter activity and BMP-4-response. Moreover, we found that the transcription factors, Oaz and PEBP2alphaA, were able to elicit additive effects with BMP-4 signaling on Xbr-1a/Xvent-2 reporter activities. These results indicate that transcriptional regulation of the Xbr-1a/Xvent-2 gene occurs in a complex mode through the cooperation of various transcription factors.

PubMed ID: 12419328
Article link: Biochem Biophys Res Commun

Species referenced: Xenopus laevis
Genes referenced: rest ventx2.1 ventx2.2 znf423