Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
EMBO J
2002 Nov 01;2121:5853-63. doi: 10.1093/emboj/cdf585.
Show Gene links
Show Anatomy links
Assisted RNP assembly: SMN and PRMT5 complexes cooperate in the formation of spliceosomal UsnRNPs.
Meister G
,
Fischer U
.
???displayArticle.abstract???
Although spliceosomal Sm proteins can assemble spontaneously onto UsnRNA in vitro, this process requires assisting factors in vivo. SMN, the protein involved in spinal muscular atrophy, is part of a complex that contains the Sm proteins and serves as a critical factor for this reaction. Here, we have reconstituted the SMN-dependent assembly of UsnRNPs in vitro. We demonstrate that the SMN complex is necessary and sufficient for the assembly reaction. The PRMT5 complex, previously implicated in methylation and storage of Sm proteins, interacts with the SMN complex and enhances its activity in an ATP-dependent manner. These data uncover the SMN-PRMT5 complex as a functional entity that promotes the assisted assembly of spliceosomal UsnRNPs, and potentially other, RNA-protein complexes.
Brahms,
Symmetrical dimethylation of arginine residues in spliceosomal Sm protein B/B' and the Sm-like protein LSm4, and their interaction with the SMN protein.
2001, Pubmed
Brahms,
Symmetrical dimethylation of arginine residues in spliceosomal Sm protein B/B' and the Sm-like protein LSm4, and their interaction with the SMN protein.
2001,
Pubmed
Bringmann,
Purification of the individual snRNPs U1, U2, U5 and U4/U6 from HeLa cells and characterization of their protein constituents.
1986,
Pubmed
Bühler,
Essential role for the tudor domain of SMN in spliceosomal U snRNP assembly: implications for spinal muscular atrophy.
1999,
Pubmed
,
Xenbase
Campbell,
Direct interaction of Smn with dp103, a putative RNA helicase: a role for Smn in transcription regulation?
2000,
Pubmed
Charroux,
Gemin3: A novel DEAD box protein that interacts with SMN, the spinal muscular atrophy gene product, and is a component of gems.
1999,
Pubmed
Charroux,
Gemin4. A novel component of the SMN complex that is found in both gems and nucleoli.
2000,
Pubmed
,
Xenbase
Fischer,
The SMN-SIP1 complex has an essential role in spliceosomal snRNP biogenesis.
1997,
Pubmed
,
Xenbase
Friesen,
Specific sequences of the Sm and Sm-like (Lsm) proteins mediate their interaction with the spinal muscular atrophy disease gene product (SMN).
2000,
Pubmed
Friesen,
SMN, the product of the spinal muscular atrophy gene, binds preferentially to dimethylarginine-containing protein targets.
2001,
Pubmed
Friesen,
The methylosome, a 20S complex containing JBP1 and pICln, produces dimethylarginine-modified Sm proteins.
2001,
Pubmed
Grundhoff,
Characterization of DP103, a novel DEAD box protein that binds to the Epstein-Barr virus nuclear proteins EBNA2 and EBNA3C.
1999,
Pubmed
Hamm,
An abundant U6 snRNP found in germ cells and embryos of Xenopus laevis.
1989,
Pubmed
,
Xenbase
Jones,
Direct interaction of the spinal muscular atrophy disease protein SMN with the small nucleolar RNA-associated protein fibrillarin.
2001,
Pubmed
,
Xenbase
Lefebvre,
Identification and characterization of a spinal muscular atrophy-determining gene.
1995,
Pubmed
Lefebvre,
Correlation between severity and SMN protein level in spinal muscular atrophy.
1997,
Pubmed
Liu,
The spinal muscular atrophy disease gene product, SMN, and its associated protein SIP1 are in a complex with spliceosomal snRNP proteins.
1997,
Pubmed
Liu,
A novel nuclear structure containing the survival of motor neurons protein.
1996,
Pubmed
,
Xenbase
Meister,
Characterization of a nuclear 20S complex containing the survival of motor neurons (SMN) protein and a specific subset of spliceosomal Sm proteins.
2000,
Pubmed
Meister,
A multiprotein complex mediates the ATP-dependent assembly of spliceosomal U snRNPs.
2001,
Pubmed
,
Xenbase
Meister,
Methylation of Sm proteins by a complex containing PRMT5 and the putative U snRNP assembly factor pICln.
2001,
Pubmed
,
Xenbase
Meister,
SMN-mediated assembly of RNPs: a complex story.
2002,
Pubmed
Monani,
The human centromeric survival motor neuron gene (SMN2) rescues embryonic lethality in Smn(-/-) mice and results in a mouse with spinal muscular atrophy.
2000,
Pubmed
Mourelatos,
SMN interacts with a novel family of hnRNP and spliceosomal proteins.
2001,
Pubmed
Pellizzoni,
SMN mutants of spinal muscular atrophy patients are defective in binding to snRNP proteins.
1999,
Pubmed
Pellizzoni,
A functional interaction between the survival motor neuron complex and RNA polymerase II.
2001,
Pubmed
Pellizzoni,
The survival of motor neurons (SMN) protein interacts with the snoRNP proteins fibrillarin and GAR1.
2001,
Pubmed
Pellizzoni,
Purification of native survival of motor neurons complexes and identification of Gemin6 as a novel component.
2002,
Pubmed
Pillai,
Purified U7 snRNPs lack the Sm proteins D1 and D2 but contain Lsm10, a new 14 kDa Sm D1-like protein.
2001,
Pubmed
Pu,
pICln inhibits snRNP biogenesis by binding core spliceosomal proteins.
1999,
Pubmed
,
Xenbase
Raker,
Spliceosomal U snRNP core assembly: Sm proteins assemble onto an Sm site RNA nonanucleotide in a specific and thermodynamically stable manner.
1999,
Pubmed
Raker,
The snRNP core assembly pathway: identification of stable core protein heteromeric complexes and an snRNP subcore particle in vitro.
1996,
Pubmed
Rossoll,
Specific interaction of Smn, the spinal muscular atrophy determining gene product, with hnRNP-R and gry-rbp/hnRNP-Q: a role for Smn in RNA processing in motor axons?
2002,
Pubmed
Schmalbruch,
Spinal muscular atrophy: present state.
2001,
Pubmed
Sendtner,
Molecular mechanisms in spinal muscular atrophy: models and perspectives.
2001,
Pubmed
Törö,
RNA binding in an Sm core domain: X-ray structure and functional analysis of an archaeal Sm protein complex.
2001,
Pubmed
Urlaub,
Sm protein-Sm site RNA interactions within the inner ring of the spliceosomal snRNP core structure.
2001,
Pubmed
Will,
Spliceosomal UsnRNP biogenesis, structure and function.
2001,
Pubmed
