XB-ART-6276Nucleic Acids Res November 1, 2002; 30 (21): 4667-74.
An analysis of the sequence requirements of EDEN-BP for specific RNA binding.
EDEN-BP (embryo deadenylation element-binding protein) binds specifically to the EDEN motif in the 3''-untranslated regions of maternal mRNAs and targets these mRNAs for deadenylation and translational repression in Xenopus laevis embryos. EDEN-BP contains three RNA recognition motifs (RRMs) and is related to the elav family of RNA-binding proteins. In the present study we show that the two N-terminal RRMs of EDEN-BP are necessary for the interaction with EDEN as well as a part of the linker region (between RRM2 and RRM3). Using a band shift assay we show that two different complexes are formed according to the size and, therefore, the functional nature of the EDEN motif. Finally, we show that EDEN-BP can form a dimer in a two-hybrid assay. Accordingly, we suggest that the functional configuration of EDEN-BP is a dimer.
PubMed ID: 12409457
PMC ID: PMC135792
Article link: Nucleic Acids Res
Species referenced: Xenopus laevis
Genes referenced: celf1 rrm2.2
References [+] :
Abe, Two different RNA binding activities for the AU-rich element and the poly(A) sequence of the mouse neuronal protein mHuC. 1997, Pubmed