Due to necessary maintenance, Xenbase will be unavailable from December 24-29, 2014. We apologize for the inconvenience.

Click on this message to dismiss it.
Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
XB-ART-6419
Cell. October 4, 2002; 111 (1): 77-89.

Asymmetries in H+/K+-ATPase and cell membrane potentials comprise a very early step in left-right patterning.

Levin M , Thorlin T , Robinson KR , Nogi T , Mercola M .


Abstract
A pharmacological screen identified the H+ and K+ ATPase transporter as obligatory for normal orientation of the left-right body axis in Xenopus. Maternal H+/K+-ATPase mRNA is symmetrically expressed in the 1-cell Xenopus embryo but becomes localized during the first two cell divisions, demonstrating that asymmetry is generated within two hours postfertilization. Although H+/K+-ATPase subunit mRNAs are symmetrically localized in chick embryos, an endogenous H+/K+-ATPase-dependent difference in membrane voltage potential exists between the left and right sides of the primitive streak. In both species, pharmacologic or genetic perturbation of endogenous H+/K+-ATPase randomized the sided pattern of asymmetrically expressed genes and induced organ heterotaxia. Thus, LR asymmetry determination depends on a very early differential ion flux created by H+/K+-ATPase activity.

PubMed ID: 12372302
Article link: Cell.
Grant support: R01 HL63271 NHLBI NIH HHS , R01 HL63271 NHLBI NIH HHS , R01 HL63271 NHLBI NIH HHS , R01 HL63271 NHLBI NIH HHS , R01 HL63271 NHLBI NIH HHS , R01 HL63271 NHLBI NIH HHS

Genes referenced: rpsa
Antibodies referenced:
Morpholinos referenced:

My Xenbase: [ Log-in / Register ]
version: [3.3]


Major funding for Xenbase is provided by the National Institute of Child Health and Human Development, grant P41 HD064556