XB-ART-6431Nat Neurosci. November 1, 2002; 5 (11): 1169-76.
A transient receptor potential channel expressed in taste receptor cells.
We used differential screening of cDNAs from individual taste receptor cells to identify candidate taste transduction elements in mice. Among the differentially expressed clones, one encoded Trpm5, a member of the mammalian family of transient receptor potential (TRP) channels. We found Trpm5 to be expressed in a restricted manner, with particularly high levels in taste tissue. In taste cells, Trpm5 was coexpressed with taste-signaling molecules such as alpha-gustducin, Ggamma13, phospholipase C-beta2 (PLC-beta2) and inositol 1,4,5-trisphosphate receptor type III (IP3R3). Our heterologous expression studies of Trpm5 indicate that it functions as a cationic channel that is gated when internal calcium stores are depleted. Trpm5 may be responsible for capacitative calcium entry in taste receptor cells that respond to bitter and/or sweet compounds.
PubMed ID: 12368808
Article link: Nat Neurosci.
Grant support: DC00310 NIDCD NIH HHS , DC03055 NIDCD NIH HHS , DC03155 NIDCD NIH HHS
Genes referenced: trpm5