Houston DW and Wylie C (2002), Cloning and expression of Xenopus Lrp5 and Lrp6...

XB-ART-6619

Mech Dev 2002 Sep 01;1171-2:337-42. doi: 10.1016/s0925-4773(02)00205-8.

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Cloning and expression of Xenopus Lrp5 and Lrp6 genes.

Houston DW , Wylie C .

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LRP5 and LRP6 comprise a subfamily of lipoprotein-receptor related proteins that function as co-receptors for Wnt proteins. Mutation of human LRP5 is responsible for osteoporosis-pseudoglioma syndrome and disruption of Lrp6 in mice causes similar effects to mutation of several different Wnt genes. We have cloned Xenopus homologues of Lrp5 and Lrp6 (Xlrp5, Xlrp6) and examined their expression during embryogenesis. Both genes are expressed maternally and ubiquitously through early development. At later stages, Xlrp5 is found in the eye, forebrain, hindbrain, branchial arches and the tip of the tail bud. Xlrp6 is expressed throughout the central nervous system, branchial arches, in the eye and otic vesicle. Both genes are also expressed at the intersomitic boundary. These results suggest roles for Wnt signaling via LRP proteins in these tissues.

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Species referenced: Xenopus laevis
Genes referenced: lrp1 lrp5 lrp6

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	Fig. 2. Expression of Xlrp5 and Xlrp6 during development. (A) Expression of Xlrp6 during oogenesis. Transcripts are uniformly expressed in early stages (II/III) and accumulate in the animal pole of stage VI oocytes (arrowhead). (B) Expression of Xlrp6 in stage 18 embryos. Staining is enriched in dorsal tissues (arrowhead). (C,C′) Xlrp5 expression in stage 28 (C) and stage 35 (C′) embryos. (D,D′) Xlrp6 expression in stage 28 (D) and stage 35 (D′) embryos. (E–G) Transverse sections at the hindbrain level through Xlrp5 (E) or Xlrp6 (F,G) stained stage 35 embryos. ba, branchial arches; ey, eye; fb, forebrain; fg, foregut; hb, hindbrain; isb, intersomite boundary; no, notochord; nt neural tube; ov, otic vesicle; p, pharynx; tb, tailbud.
	lrp5 (low density lipoprotein receptor-related protein 5) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 28, lateral view, anterior left, dorsal up.
	lrp6 (low density lipoprotein receptor-related protein 6) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 35, lateral view, anterior left, dorsal up.