Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
XB-ART-6624
Mech Dev September 1, 2002; 117 (1-2): 103-13.

A screen for co-factors of Six3.

Tessmar K , Loosli F , Wittbrodt J .


Abstract
The vertebrate Six3 gene a homeobox gene of the Six-family, plays a crucial role in early eye and forebrain development. Here we report the isolation of candidate factors that interact with Six3 in a yeast two-hybrid screen. Among these are two basic helix loop helix (bHLH) domain containing proteins. Biochemical analysis reveals that the bHLH proteins ATH5, ATH3, NEUROD as well as ASH1 interact specifically with XSix3. By defining the interacting domains we show that the bHLH domain of NEUROD interacts with the SIX domain of XSix3. The co-expression of the interacting molecules during late retina determination/differentiation suggests a new role for Six3 and the respective interaction partner also in these late steps of eye development.

PubMed ID: 12204251
Article link: Mech Dev

Genes referenced: actl6a arntl2 ascl1 atoh7 atp5if1 chmp1a cldn6.2 neurod1 neurod4 scoc six3 six6 smarcc1 strn tacc3 tle2 znf326


Article Images: [+] show captions


Xenbase: The Xenopus Model Organism Knowledgebase.
Version: 4.16.0
Major funding for Xenbase is provided by grant P41 HD064556