Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Intranuclear degradation of nonsense codon-containing mRNA.
Bühler M
,
Wilkinson MF
,
Mühlemann O
.
???displayArticle.abstract???
Most vertebrate mRNAs with premature termination codons (PTCs) are specifically recognized and degraded by a process referred to as nonsense-mediated mRNA decay (NMD) while still associated with the nucleus. However, it is still a matter of debate whether PTCs can be identified by intranuclear scanning or only by ribosomes on the cytoplasmic side of the nuclear envelope. Here we show that inhibition of mRNA export by two independent approaches does not affect the downregulation of PTC-containing T-cell receptor beta transcripts in the nuclear fraction of mammalian cells, providing strong evidence for intranuclear NMD. Our results are fully consistent with recently reported evidence for nuclear translation and suggest that an important biological role for nuclear ribosomes is the early elimination of nonsense mRNA during a pioneer round of translation.
Ahmed,
Effect of vesicular stomatitis virus matrix protein on transcription directed by host RNA polymerases I, II, and III.
1998, Pubmed
Ahmed,
Effect of vesicular stomatitis virus matrix protein on transcription directed by host RNA polymerases I, II, and III.
1998,
Pubmed
Aoufouchi,
Nonsense mutations inhibit RNA splicing in a cell-free system: recognition of mutant codon is independent of protein synthesis.
1996,
Pubmed
Baserga,
Beta-globin nonsense mutation: deficient accumulation of mRNA occurs despite normal cytoplasmic stability.
1992,
Pubmed
Belgrader,
Evidence to implicate translation by ribosomes in the mechanism by which nonsense codons reduce the nuclear level of human triosephosphate isomerase mRNA.
1993,
Pubmed
Belgrader,
Nonsense but not missense mutations can decrease the abundance of nuclear mRNA for the mouse major urinary protein, while both types of mutations can facilitate exon skipping.
1994,
Pubmed
Belgrader,
Mammalian nonsense codons can be cis effectors of nuclear mRNA half-life.
1994,
Pubmed
Brogna,
Pre-mRNA processing: insights from nonsense.
2001,
Pubmed
Carneiro,
Accumulation of rare and moderately abundant mRNAs in mouse L-cells is mainly post-transcriptionally regulated.
1984,
Pubmed
Carter,
A regulatory mechanism that detects premature nonsense codons in T-cell receptor transcripts in vivo is reversed by protein synthesis inhibitors in vitro.
1995,
Pubmed
Carter,
A splicing-dependent regulatory mechanism that detects translation signals.
1996,
Pubmed
Cheng,
Nonsense codons can reduce the abundance of nuclear mRNA without affecting the abundance of pre-mRNA or the half-life of cytoplasmic mRNA.
1993,
Pubmed
Fontoura,
A conserved biogenesis pathway for nucleoporins: proteolytic processing of a 186-kilodalton precursor generates Nup98 and the novel nucleoporin, Nup96.
1999,
Pubmed
Frischmeyer,
Nonsense-mediated mRNA decay in health and disease.
1999,
Pubmed
Gatfield,
The DExH/D box protein HEL/UAP56 is essential for mRNA nuclear export in Drosophila.
2001,
Pubmed
Hentze,
A perfect message: RNA surveillance and nonsense-mediated decay.
1999,
Pubmed
Her,
Inhibition of Ran guanosine triphosphatase-dependent nuclear transport by the matrix protein of vesicular stomatitis virus.
1997,
Pubmed
,
Xenbase
Hilleren,
Mechanisms of mRNA surveillance in eukaryotes.
1999,
Pubmed
Iborra,
Coupled transcription and translation within nuclei of mammalian cells.
2001,
Pubmed
Ishigaki,
Evidence for a pioneer round of mRNA translation: mRNAs subject to nonsense-mediated decay in mammalian cells are bound by CBP80 and CBP20.
2001,
Pubmed
Jensen,
The DECD box putative ATPase Sub2p is an early mRNA export factor.
2001,
Pubmed
Kessler,
Effects of nonsense mutations on nuclear and cytoplasmic adenine phosphoribosyltransferase RNA.
1996,
Pubmed
Kopecky,
Matrix protein and another viral component contribute to induction of apoptosis in cells infected with vesicular stomatitis virus.
2001,
Pubmed
Li,
T cell receptor (TCR) mini-gene mRNA expression regulated by nonsense codons: a nuclear-associated translation-like mechanism.
1997,
Pubmed
Li,
Nonsense surveillance in lymphocytes?
1998,
Pubmed
Lozano,
Low cytoplasmic mRNA levels of immunoglobulin kappa light chain genes containing nonsense codons correlate with inefficient splicing.
1994,
Pubmed
Luo,
Pre-mRNA splicing and mRNA export linked by direct interactions between UAP56 and Aly.
2001,
Pubmed
,
Xenbase
Maquat,
When cells stop making sense: effects of nonsense codons on RNA metabolism in vertebrate cells.
1995,
Pubmed
McKendrick,
Interaction of eukaryotic translation initiation factor 4G with the nuclear cap-binding complex provides a link between nuclear and cytoplasmic functions of the m(7) guanosine cap.
2001,
Pubmed
Moore,
Nuclear RNA turnover.
2002,
Pubmed
Mühlemann,
Precursor RNAs harboring nonsense codons accumulate near the site of transcription.
2001,
Pubmed
Noensie,
A strategy for disease gene identification through nonsense-mediated mRNA decay inhibition.
2001,
Pubmed
Petersen,
The matrix protein of vesicular stomatitis virus inhibits nucleocytoplasmic transport when it is in the nucleus and associated with nuclear pore complexes.
2000,
Pubmed
,
Xenbase
Radu,
The peptide repeat domain of nucleoporin Nup98 functions as a docking site in transport across the nuclear pore complex.
1995,
Pubmed
Strässer,
Splicing factor Sub2p is required for nuclear mRNA export through its interaction with Yra1p.
2001,
Pubmed
Thermann,
Binary specification of nonsense codons by splicing and cytoplasmic translation.
1998,
Pubmed
Urlaub,
Nonsense mutations in the dihydrofolate reductase gene affect RNA processing.
1989,
Pubmed
Wilkinson,
RNA surveillance by nuclear scanning?
2002,
Pubmed
Wilusz,
Curbing the nonsense: the activation and regulation of mRNA surveillance.
2001,
Pubmed
von Kobbe C,
Vesicular stomatitis virus matrix protein inhibits host cell gene expression by targeting the nucleoporin Nup98.
2000,
Pubmed