XB-ART-722Science. February 10, 2006; 311 (5762): 856-61.
The nucleosomal surface as a docking station for Kaposi''s sarcoma herpesvirus LANA.
Kaposi''s sarcoma-associated herpesvirus (KSHV) latency-associated nuclear antigen (LANA) mediates viral genome attachment to mitotic chromosomes. We find that N-terminal LANA docks onto chromosomes by binding nucleosomes through the folded region of histones H2A-H2B. The same LANA residues were required for both H2A-H2B binding and chromosome association. Further, LANA did not bind Xenopus sperm chromatin, which is deficient in H2A-H2B; chromatin binding was rescued after assembly of nucleosomes containing H2A-H2B. We also describe the 2.9-angstrom crystal structure of a nucleosome complexed with the first 23 LANA amino acids. The LANA peptide forms a hairpin that interacts exclusively with an acidic H2A-H2B region that is implicated in the formation of higher order chromatin structure. Our findings present a paradigm for how nucleosomes may serve as binding platforms for viral and cellular proteins and reveal a previously unknown mechanism for KSHV latency.
PubMed ID: 16469929
Article link: Science.
Grant support: CA82036 NCI NIH HHS , GM067777 NIGMS NIH HHS , GM62267 NIGMS NIH HHS , R01 GM067777-05 NIGMS NIH HHS , R01 GM067777 NIGMS NIH HHS
Genes referenced: hist2h2ab hist2h2be