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EMBO Rep
2002 May 01;35:457-62. doi: 10.1093/embo-reports/kvf095.
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APC/Fizzy-Related targets Aurora-A kinase for proteolysis.
Castro A
,
Arlot-Bonnemains Y
,
Vigneron S
,
Labbé JC
,
Prigent C
,
Lorca T
.
???displayArticle.abstract??? Aurora-A kinase is a mitotic spindle-pole-associated protein that has been implicated in duplication and separation of centrosomes and in spindle assembly. The proper timing and amplitude of Aurora-A expression seems to be important, as elevated levels of this protein have been associated with centrosome abnormalities and aneuploidy in mammalian cells. We show that Aurora-A increases at the G2-M transistion and disappears completely at G1 in XL2 cells. Using Xenopus oocyte extracts, we demonstrate that degradation of Aurora-A is mediated by the anaphase-promoting complex (APC) and is regulated by Fizzy-Related but not by Fizzy. Degradation of Aurora-A depends on a D-Box, but not on its KEN-Box motif, as mutation of its C-terminal D-Box sequence induces stabilization of the protein. Accordingly, addition into the extracts of a cyclin B-type D-Box-motif-containing peptide completely suppresses its degradation. Furthermore, APC/Fizzy-Related ubiquitylates the wild type but not a D-Box mutant form of Aurora-A in vitro. Consistent with these data, ectopic expression of Fizzy-Related in Xenopus oocytes induces complete degradation of endogenous Aurora-A. Aurora-A is thus the first protein, at least in our assay system, that undergoes a D-Box-dependent degradation mediated by APC/Fizzy-Related but not by APC/Fizzy.
Arlot-Bonnemains,
Identification of a functional destruction box in the Xenopus laevis aurora-A kinase pEg2.
2001, Pubmed,
Xenbase
Arlot-Bonnemains,
Identification of a functional destruction box in the Xenopus laevis aurora-A kinase pEg2.
2001,
Pubmed
,
Xenbase
Bembenek,
Regulation of the anaphase-promoting complex by the dual specificity phosphatase human Cdc14a.
2001,
Pubmed
Bischoff,
A homologue of Drosophila aurora kinase is oncogenic and amplified in human colorectal cancers.
1998,
Pubmed
Blanco,
APC(ste9/srw1) promotes degradation of mitotic cyclins in G(1) and is inhibited by cdc2 phosphorylation.
2000,
Pubmed
Castro,
Cyclin B/cdc2 induces c-Mos stability by direct phosphorylation in Xenopus oocytes.
2001,
Pubmed
,
Xenbase
Cohen-Fix,
Anaphase initiation in Saccharomyces cerevisiae is controlled by the APC-dependent degradation of the anaphase inhibitor Pds1p.
1996,
Pubmed
,
Xenbase
Fang,
Direct binding of CDC20 protein family members activates the anaphase-promoting complex in mitosis and G1.
1998,
Pubmed
,
Xenbase
Fesquet,
Is Cdk7/cyclin H/MAT1 the genuine cdk activating kinase in cycling Xenopus egg extracts?
1997,
Pubmed
,
Xenbase
Frank-Vaillant,
Progesterone regulates the accumulation and the activation of Eg2 kinase in Xenopus oocytes.
2000,
Pubmed
,
Xenbase
Hames,
APC/C-mediated destruction of the centrosomal kinase Nek2A occurs in early mitosis and depends upon a cyclin A-type D-box.
2001,
Pubmed
Honda,
Degradation of human Aurora2 protein kinase by the anaphase-promoting complex-ubiquitin-proteasome pathway.
2000,
Pubmed
Jaspersen,
Inhibitory phosphorylation of the APC regulator Hct1 is controlled by the kinase Cdc28 and the phosphatase Cdc14.
1999,
Pubmed
Kimura,
Cell cycle-dependent expression and spindle pole localization of a novel human protein kinase, Aik, related to Aurora of Drosophila and yeast Ipl1.
1997,
Pubmed
,
Xenbase
Kramer,
Mitotic regulation of the APC activator proteins CDC20 and CDH1.
2000,
Pubmed
,
Xenbase
Lorca,
Fizzy is required for activation of the APC/cyclosome in Xenopus egg extracts.
1998,
Pubmed
,
Xenbase
Pfleger,
The KEN box: an APC recognition signal distinct from the D box targeted by Cdh1.
2000,
Pubmed
,
Xenbase
Roghi,
The Xenopus protein kinase pEg2 associates with the centrosome in a cell cycle-dependent manner, binds to the spindle microtubules and is involved in bipolar mitotic spindle assembly.
1998,
Pubmed
,
Xenbase
Sigrist,
Drosophila fizzy-related down-regulates mitotic cyclins and is required for cell proliferation arrest and entry into endocycles.
1997,
Pubmed
,
Xenbase
Visintin,
CDC20 and CDH1: a family of substrate-specific activators of APC-dependent proteolysis.
1997,
Pubmed
Zachariae,
Whose end is destruction: cell division and the anaphase-promoting complex.
1999,
Pubmed
Zhou,
Tumour amplified kinase STK15/BTAK induces centrosome amplification, aneuploidy and transformation.
1998,
Pubmed
