FEBS Lett 2002 Apr 10;5161-3:187-90. doi: 10.1016/s0014-5793(02)02554-1.

Conventional protein kinase C isoforms regulate human dopamine transporter activity in Xenopus oocytes.

???displayArticle.abstract???
The hypothesis that specific protein kinase C (PKC) isoforms regulate dopamine transporter (DAT) function was tested in Xenopus laevis oocytes expressing human (h)DAT. Activation of conventional PKCs (cPKCs) and novel PKCs (nPKCs) using 10 nM phorbol 12-myristate 13-acetate (PMA) significantly inhibited DAT-associated transport currents. This effect was reversed by isoform-non-selective PKC inhibitors, selective inhibitors of cPKCs and deltaPKC, and by Ca2+ chelation. By contrast, the epsilonPKC translocation inhibitor peptide had no effect on PMA-induced inhibition of hDAT transport-associated currents. Thus, the primary mechanism by which PMA regulates hDAT expressed in oocytes appears to be by activating cPKC(s).

???displayArticle.pubmedLink??? 11959130
???displayArticle.link??? FEBS Lett
???displayArticle.grants??? [+]

Species referenced: Xenopus laevis
Genes referenced: slc6a3