Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Proc Natl Acad Sci U S A
2002 Mar 19;996:3968-73. doi: 10.1073/pnas.052017299.
Show Gene links
Show Anatomy links
Ionic dependence of Ca2+ channel modulation by syntaxin 1A.
Wiser O
,
Cohen R
,
Atlas D
.
???displayArticle.abstract???
Alteration of the kinetic properties of voltage-gated Ca(2+) channels, Ca(v)1.2 (Lc-type), Ca(v)2.2 (N type), and Ca(v)2.3 (R type), by syntaxin 1A (Syn1A) and synaptotagmin could modulate exocytosis. We tested how switching divalent charge carriers from Ca(2+) to Sr(2+) and Ba(2+) affected Syn1A and synaptotagmin modulation of Ca(2+)-channel activation. Syn1A accelerated Ca(v)1.2 activation if Ca(2+) was the charge carrier; and by substituting for Ba(2+), Syn1A slowed Ca(v)1.2 activation. Syn1A also significantly accelerated Ca(v)2.3 activation in Ca(2+) and marginally in Ba(2+). Synaptotagmin, on the other hand, increased the rate of activation of Ca(v)2.3 and Ca(v)2.2 in all permeating ions tested. The Syn1A-channel interaction, unlike the synaptotagmin-channel interaction, proved significantly more sensitive to the type of permeating ion. It is well established that exocytosis is affected by switching the charge carriers. Based on the present results, we suggest that the channel-Syn1A interaction could respond to the conformational changes induced within the channel during membrane depolarization and divalent ion binding. These changes could partially account for the charge specificity of synaptic transmission as well as for the fast signaling between the Ca(2+) source and the fusion apparatus of channel-associated-vesicles (CAV). Furthermore, propagation of conformational changes induced by the divalent ions appear to affect the concerted interaction of the channel with the fusion/docking machinery upstream to free Ca(2+) buildup and/or binding to a cytosolic Ca(2+) sensor. These results raise the intriguing possibility that the channel is the Ca(2+) sensor in the process of fast neurotransmitter release.
Abdul-Ghani,
Sr2+ and quantal events at excitatory synapses between mouse hippocampal neurons in culture.
1996, Pubmed
Abdul-Ghani,
Sr2+ and quantal events at excitatory synapses between mouse hippocampal neurons in culture.
1996,
Pubmed
Albillos,
R-Type Ca2+ channels are coupled to the rapid component of secretion in mouse adrenal slice chromaffin cells.
2000,
Pubmed
Augustine,
Divalent cations differentially support transmitter release at the squid giant synapse.
1984,
Pubmed
Bain,
Quantal transmitter release mediated by strontium at the mouse motor nerve terminal.
1992,
Pubmed
Barg,
Fast exocytosis with few Ca(2+) channels in insulin-secreting mouse pancreatic B cells.
2001,
Pubmed
Bertram,
Single-domain/bound calcium hypothesis of transmitter release and facilitation.
1996,
Pubmed
Bezprozvanny,
Functional impact of syntaxin on gating of N-type and Q-type calcium channels.
1995,
Pubmed
,
Xenbase
Bezprozvanny,
Molecular determinants of the functional interaction between syntaxin and N-type Ca2+ channel gating.
2000,
Pubmed
,
Xenbase
Byerly,
Permeation and interaction of divalent cations in calcium channels of snail neurons.
1985,
Pubmed
Capogna,
Ca2+ or Sr2+ partially rescues synaptic transmission in hippocampal cultures treated with botulinum toxin A and C, but not tetanus toxin.
1997,
Pubmed
Charvin,
Direct interaction of the calcium sensor protein synaptotagmin I with a cytoplasmic domain of the alpha1A subunit of the P/Q-type calcium channel.
1997,
Pubmed
Dineley,
Leitmotifs in the biochemistry of LTP induction: amplification, integration and coordination.
2001,
Pubmed
Dodge,
Co-operative action a calcium ions in transmitter release at the neuromuscular junction.
1967,
Pubmed
Goda,
Two components of transmitter release at a central synapse.
1994,
Pubmed
Heinemann,
Calcium channel characteristics conferred on the sodium channel by single mutations.
1992,
Pubmed
,
Xenbase
Hess,
Mechanism of ion permeation through calcium channels.
,
Pubmed
Kim,
Structural determinants of ion selectivity in brain calcium channel.
1993,
Pubmed
,
Xenbase
Kuo,
G-protein modulation of ion permeation through N-type calcium channels.
1993,
Pubmed
McNaughton,
Electrophysiological properties of the human N-type Ca2+ channel: I. Channel gating in Ca2+, Ba2+ and Sr2+ containing solutions.
1997,
Pubmed
Meiri,
Activation of transmitter release by strontium and calcium ions at the neuromuscular junction.
1971,
Pubmed
Mellow,
Effects of calcium and strontium in the process of acetylcholine release from motor nerve endings.
1982,
Pubmed
Miledi,
The action of calcium on neuronal synapses in the squid.
1966,
Pubmed
Miledi,
Strontium as a substitute for calcium in the process of transmitter release at the neuromuscular junction.
1966,
Pubmed
Mochida,
Inhibition of neurotransmission by peptides containing the synaptic protein interaction site of N-type Ca2+ channels.
1996,
Pubmed
Ohmori,
Surface potential reflected in both gating and permeation mechanisms of sodium and calcium channels of the tunicate egg cell membrane.
1977,
Pubmed
Prod'hom,
Interactions of protons with single open L-type calcium channels. Location of protonation site and dependence of proton-induced current fluctuations on concentration and species of permeant ion.
1989,
Pubmed
Rettig,
Isoform-specific interaction of the alpha1A subunits of brain Ca2+ channels with the presynaptic proteins syntaxin and SNAP-25.
1996,
Pubmed
Rumpel,
Sr2+-dependent asynchronous evoked transmission at rat striatal inhibitory synapses in vitro.
1999,
Pubmed
Saimi,
Are ions involved in the gating of calcium channels?
1982,
Pubmed
Sather,
Structural basis of ion channel permeation and selectivity.
1994,
Pubmed
Schneider,
Molecular analysis and functional expression of the human type E neuronal Ca2+ channel alpha 1 subunit.
1994,
Pubmed
,
Xenbase
Sheng,
Identification of a syntaxin-binding site on N-type calcium channels.
1994,
Pubmed
Sheng,
Calcium-dependent interaction of N-type calcium channels with the synaptic core complex.
1996,
Pubmed
Sheng,
Interaction of the synprint site of N-type Ca2+ channels with the C2B domain of synaptotagmin I.
1997,
Pubmed
Tang,
Molecular localization of ion selectivity sites within the pore of a human L-type cardiac calcium channel.
1993,
Pubmed
Tobi,
N-type voltage-sensitive calcium channel interacts with syntaxin, synaptotagmin and SNAP-25 in a multiprotein complex.
1998,
Pubmed
,
Xenbase
Trus,
The transmembrane domain of syntaxin 1A negatively regulates voltage-sensitive Ca(2+) channels.
2001,
Pubmed
,
Xenbase
Weiss,
Divalent cations effectively replace Ca2+ and support bradykinin induced noradrenaline release.
1990,
Pubmed
Wigström,
Strontium supports synaptic transmission and long-lasting potentiation in the hippocampus.
1980,
Pubmed
Wiser,
Functional interaction of syntaxin and SNAP-25 with voltage-sensitive L- and N-type Ca2+ channels.
1996,
Pubmed
,
Xenbase
Wiser,
The voltage sensitive Lc-type Ca2+ channel is functionally coupled to the exocytotic machinery.
1999,
Pubmed
,
Xenbase
Wiser,
Synaptotagmin restores kinetic properties of a syntaxin-associated N-type voltage sensitive calcium channel.
1997,
Pubmed
,
Xenbase
Wu,
R-type Ca2+ currents evoke transmitter release at a rat central synapse.
1998,
Pubmed
Xu-Friedman,
Presynaptic strontium dynamics and synaptic transmission.
1999,
Pubmed
Yang,
Molecular determinants of Ca2+ selectivity and ion permeation in L-type Ca2+ channels.
1993,
Pubmed
,
Xenbase
Yang,
Syntaxin 1 interacts with the L(D) subtype of voltage-gated Ca(2+) channels in pancreatic beta cells.
1999,
Pubmed
Zamponi,
Evidence for a specific site for modulation of calcium channel activation by external calcium ions.
1996,
Pubmed
,
Xenbase
Zhong,
Reciprocal regulation of P/Q-type Ca2+ channels by SNAP-25, syntaxin and synaptotagmin.
1999,
Pubmed
Zhou,
Surface charge and calcium channel saturation in bullfrog sympathetic neurons.
1995,
Pubmed
