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Biochem J
2002 Mar 01;362Pt 2:375-82. doi: 10.1042/0264-6021:3620375.
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Phosphorylation of Xenopus transcription factor IIIA by an oocyte protein kinase CK2.
Westmark CJ
,
Ghose R
,
Huber PW
.
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Transcription factor IIIA (TFIIIA), isolated from the cytoplasmic 7 S ribonucleoprotein complex of Xenopus oocytes, is phosphorylated when incubated with [gamma-(32)P]ATP. This modification is due to a trace kinase activity that remains associated with the factor through several steps of purification. The kinase can use either ATP or GTP, and will phosphorylate casein and phosvitin to the exclusion of TFIIIA. The kinase is reactive with a ten-amino-acid peptide that is a specific substrate for protein kinase CK2 (CK2; formerly casein kinase II). In addition, inhibition of phosphorylation by heparin and stimulation by spermidine indicate that the activity can be ascribed to CK2. Phospho amino acid analysis established that serine is the sole phosphoryl acceptor in TFIIIA. There are four consensus sites for CK2 in TFIIIA; all contain serine residues at the putative site of phosphorylation. TFIIIA immunoprecipitated from oocytes, which were incubated with [(32)P]orthophosphate, is also phosphorylated exclusively on serine residues. Only the cyanogen bromide fragment, which was derived from the N-terminal end of TFIIIA, is labelled in vivo. A recognition sequence for CK2, located at Ser(16) in the beta-turn of the first zinc-finger domain, is the only protein kinase consensus sequence present in this peptide. Assays in vitro with site-specific mutants of TFIIIA established that Ser(16) is the preferred site of phosphorylation, with some secondary modification at Ser(314).
Allende,
Protein kinases. 4. Protein kinase CK2: an enzyme with multiple substrates and a puzzling regulation.
1995, Pubmed
Allende,
Protein kinases. 4. Protein kinase CK2: an enzyme with multiple substrates and a puzzling regulation.
1995,
Pubmed
Bieker,
Physical properties and DNA-binding stoichiometry of a 5 S gene-specific transcription factor.
1984,
Pubmed
,
Xenbase
Birkenmeier,
A nuclear extract of Xenopus laevis oocytes that accurately transcribes 5S RNA genes.
1978,
Pubmed
,
Xenbase
Bradford,
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
1976,
Pubmed
Brown,
RNA polymerase III transcription: its control by tumor suppressors and its deregulation by transforming agents.
2000,
Pubmed
Chipev,
Chromosomal organization of Xenopus laevis oocyte and somatic 5S rRNA genes in vivo.
1992,
Pubmed
,
Xenbase
Chomczynski,
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
1987,
Pubmed
Conesa,
On the subunit composition, stoichiometry, and phosphorylation of the yeast transcription factor TFIIIC/tau.
1993,
Pubmed
Cooper,
Detection and quantification of phosphotyrosine in proteins.
1983,
Pubmed
Cummings,
Protein kinase activity associated with stored messenger ribonucleoprotein particles of Xenopus oocytes.
1988,
Pubmed
,
Xenbase
Del Río,
High yield purification of active transcription factor IIIA expressed in E. coli.
1991,
Pubmed
,
Xenbase
Deschamps,
mRNP3 and mRNP4 are phosphorylatable by casein kinase II in Xenopus oocytes, but phosphorylation does not modify RNA-binding affinity.
1997,
Pubmed
,
Xenbase
Dumont,
Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals.
1972,
Pubmed
,
Xenbase
Engelke,
Chromosomal footprinting of transcriptionally active and inactive oocyte-type 5S RNA genes of Xenopus laevis.
1990,
Pubmed
,
Xenbase
Engelke,
Specific interaction of a purified transcription factor with an internal control region of 5S RNA genes.
1980,
Pubmed
,
Xenbase
Fan,
Phosphorylation of the human La antigen on serine 366 can regulate recycling of RNA polymerase III transcription complexes.
1997,
Pubmed
Gatica,
Nucleic acids can regulate the activity of casein kinase II.
1989,
Pubmed
,
Xenbase
Ghavidel,
Casein kinase II regulation of yeast TFIIIB is mediated by the TATA-binding protein.
1997,
Pubmed
Ghavidel,
A review of progress towards elucidating the role of protein kinase CK2 in polymerase III transcription: regulation of the TATA binding protein.
1999,
Pubmed
Glikin,
Chromatin assembly in Xenopus oocytes: in vitro studies.
1984,
Pubmed
,
Xenbase
Gottesfeld,
Mitotic repression of RNA polymerase III transcription in vitro mediated by phosphorylation of a TFIIIB component.
1994,
Pubmed
,
Xenbase
Gottesfeld,
Mitotic repression of the transcriptional machinery.
1997,
Pubmed
Hanas,
Cooperative model for the binding of Xenopus transcription factor A to the 5S RNA gene.
1983,
Pubmed
,
Xenbase
Hazuda,
DNA-activated ATPase activity associated with Xenopus transcription factor A.
1986,
Pubmed
,
Xenbase
Hockman,
Casein kinase II is required for efficient transcription by RNA polymerase III.
1996,
Pubmed
Hoeffler,
Activation of transcription factor IIIC by the adenovirus E1A protein.
1988,
Pubmed
Holcomb,
Phosphorylation of the C-proteins of HeLa cell hnRNP particles. Involvement of a casein kinase II-type enzyme.
1984,
Pubmed
Honda,
Association of a 5S gene transcription factor with 5S RNA and altered levels of the factor during cell differentiation.
1980,
Pubmed
,
Xenbase
Huber,
Nuclease protection analysis of ribonucleoprotein complexes: use of the cytotoxic ribonuclease alpha-sarcin to determine the binding sites for Escherichia coli ribosomal proteins L5, L18, and L25 on 5S rRNA.
1984,
Pubmed
Kandror,
RNA-binding protein kinase from amphibian oocytes is a casein kinase II.
1984,
Pubmed
Kim,
Interaction of the beta subunit of casein kinase II with the ribosomal protein L5.
1996,
Pubmed
Kuchino,
Analysis of modified nucleosides and nucleotide sequence of tRNA.
1987,
Pubmed
Kuenzel,
A synthetic peptide substrate specific for casein kinase II.
1985,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Lassar,
Transcription of class III genes: formation of preinitiation complexes.
1983,
Pubmed
,
Xenbase
McConkey,
TFIIIA binds with equal affinity to somatic and major oocyte 5S RNA genes.
1988,
Pubmed
,
Xenbase
McDonald,
Site and consequences of the autophosphorylation of Ca2+/calmodulin-dependent protein kinase type "Gr".
1993,
Pubmed
Mulner-Lorillon,
Purification and characterization of a casein-kinase-II-type enzyme from Xenopus laevis ovary. Biological effects on the meiotic cell division of full-grown oocyte.
1988,
Pubmed
,
Xenbase
Park,
Phosphorylation of ribosomal protein L5 by protein kinase CKII decreases its 5S rRNA binding activity.
1999,
Pubmed
Pelham,
A specific transcription factor that can bind either the 5S RNA gene or 5S RNA.
1980,
Pubmed
,
Xenbase
Pinna,
Casein kinase 2: an 'eminence grise' in cellular regulation?
1990,
Pubmed
Rawlings,
Analysis of the binding of Xenopus transcription factor IIIA to oocyte 5 S rRNA and to the 5 S rRNA gene.
1996,
Pubmed
,
Xenbase
Reynolds,
The tyrosine phosphatase cdc25 selectively inhibits transcription of the Xenopus oocyte-type tRNAtyrC gene.
1993,
Pubmed
,
Xenbase
Schägger,
Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa.
1987,
Pubmed
Shastry,
Altered levels of a 5 S gene-specific transcription factor (TFIIIA) during oogenesis and embryonic development of Xenopus laevis.
1984,
Pubmed
,
Xenbase
Smith,
Domains of the positive transcription factor specific for the Xenopus 5S RNA gene.
1984,
Pubmed
,
Xenbase
Stepanov,
Protein kinase activity in RNA-binding proteins of Amphibia oocytes.
1982,
Pubmed
,
Xenbase
Taylor,
The developmental expression of the gene for TFIIIA in Xenopus laevis.
1986,
Pubmed
,
Xenbase
White,
Regulation of RNA polymerase III transcription in response to Simian virus 40 transformation.
1990,
Pubmed
Whitmarsh,
Regulation of transcription factor function by phosphorylation.
2000,
Pubmed
Wilhelm,
Expression of the subunits of protein kinase CK2 during oogenesis in Xenopus laevis.
1995,
Pubmed
,
Xenbase
Wolffe,
Differential 5S RNA gene expression in vitro.
1987,
Pubmed
,
Xenbase
Wolffe,
Transcription fraction TFIIIC can regulate differential Xenopus 5S RNA gene transcription in vitro.
1988,
Pubmed
,
Xenbase
Wolffe,
Developmental regulation of two 5S ribosomal RNA genes.
1988,
Pubmed
,
Xenbase
