Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Plant Physiol
2002 Feb 01;1282:400-10. doi: 10.1104/pp.010832.
Show Gene links
Show Anatomy links
Electrophysiological analysis of cloned cyclic nucleotide-gated ion channels.
Leng Q
,
Mercier RW
,
Hua BG
,
Fromm H
,
Berkowitz GA
.
???displayArticle.abstract???
Electrophysiological studies were conducted on the cloned plant cyclic nucleotide-gated ion channels AtCNGC2 and AtCNGC1 from Arabidopsis, and NtCBP4 from tobacco (Nicotiana tobacum). The nucleotide coding sequences for these proteins were expressed in Xenopus laevis oocytes or HEK 293 cells. Channel characteristics were evaluated using voltage clamp analysis of currents in the presence of cAMP. AtCNGC2 was demonstrated to conduct K(+) and other monovalent cations, but exclude Na(+); this conductivity profile is unique for any ion channel not possessing the amino acid sequence found in the selectivity filter of K(+)-selective ion channels. Application of cAMP evoked currents in membrane patches of oocytes injected with AtCNGC2 cRNA. Direct activation of the channel by cyclic nucleotide, demonstrated by application of cyclic nucleotide to patches of membranes expressing such channels, is a hallmark characteristic of this ion channel family. Voltage clamp studies (two-electrode configuration) demonstrated that AtCNGC1 and NtCBP4 are also cyclic nucleotide-gated channels. Addition of a lipophilic analog of cAMP to the perfusion bath of oocytes injected with NtCBP4 and AtCNGC1 cRNAs induced inward rectified, noninactivating K(+) currents.
Anderson,
Functional expression of a probable Arabidopsis thaliana potassium channel in Saccharomyces cerevisiae.
1992, Pubmed
Anderson,
Functional expression of a probable Arabidopsis thaliana potassium channel in Saccharomyces cerevisiae.
1992,
Pubmed
Arazi,
A tobacco plasma membrane calmodulin-binding transporter confers Ni2+ tolerance and Pb2+ hypersensitivity in transgenic plants.
1999,
Pubmed
Arazi,
A high-affinity calmodulin-binding site in a tobacco plasma-membrane channel protein coincides with a characteristic element of cyclic nucleotide-binding domains.
2000,
Pubmed
Biel,
Structure and function of cyclic nucleotide-gated channels.
1999,
Pubmed
Biel,
Cyclic nucleotide gated channels.
1999,
Pubmed
Brüggemann,
Ether-à-go-go encodes a voltage-gated channel permeable to K+ and Ca2+ and modulated by cAMP.
1993,
Pubmed
,
Xenbase
Clough,
The Arabidopsis dnd1 "defense, no death" gene encodes a mutated cyclic nucleotide-gated ion channel.
2000,
Pubmed
Crary,
Mutation of a single residue in the S2-S3 loop of CNG channels alters the gating properties and sensitivity to inhibitors.
2000,
Pubmed
,
Xenbase
Doyle,
The structure of the potassium channel: molecular basis of K+ conduction and selectivity.
1998,
Pubmed
Eismann,
A single negative charge within the pore region of a cGMP-gated channel controls rectification, Ca2+ blockage, and ionic selectivity.
1994,
Pubmed
,
Xenbase
Finn,
Cyclic nucleotide-gated ion channels: an extended family with diverse functions.
1996,
Pubmed
Heginbotham,
Mutations in the K+ channel signature sequence.
1994,
Pubmed
,
Xenbase
Hoshi,
Regulation of voltage dependence of the KAT1 channel by intracellular factors.
1995,
Pubmed
,
Xenbase
Immke,
Influence of non-P region domains on selectivity filter properties in voltage-gated K+ channels.
1998,
Pubmed
Jurman,
Visual identification of individual transfected cells for electrophysiology using antibody-coated beads.
1994,
Pubmed
Köhler,
Developmentally regulated expression of a cyclic nucleotide-gated ion channel from Arabidopsis indicates its involvement in programmed cell death.
2001,
Pubmed
Köhler,
Characterisation of calmodulin binding to cyclic nucleotide-gated ion channels from Arabidopsis thaliana.
2000,
Pubmed
Köhler,
Characterisation of a novel gene family of putative cyclic nucleotide- and calmodulin-regulated ion channels in Arabidopsis thaliana.
1999,
Pubmed
Lacombe,
The identity of plant glutamate receptors.
2001,
Pubmed
Lang,
KCNA10: a novel ion channel functionally related to both voltage-gated potassium and CNG cation channels.
2000,
Pubmed
,
Xenbase
Lee,
Electrophysiological characteristics of rat gustatory cyclic nucleotide--gated channel expressed in Xenopus oocytes.
2001,
Pubmed
,
Xenbase
Leng,
Cloning and first functional characterization of a plant cyclic nucleotide-gated cation channel.
1999,
Pubmed
,
Xenbase
Li,
Cyclic AMP stimulates K+ channel activity in mesophyll cells of Vicia faba L.
1994,
Pubmed
Liman,
Subunit stoichiometry of a mammalian K+ channel determined by construction of multimeric cDNAs.
1992,
Pubmed
,
Xenbase
Lingueglia,
Expression cloning of an epithelial amiloride-sensitive Na+ channel. A new channel type with homologies to Caenorhabditis elegans degenerins.
1993,
Pubmed
,
Xenbase
Mäser,
Phylogenetic relationships within cation transporter families of Arabidopsis.
2001,
Pubmed
Müller-Röber,
Cloning and electrophysiological analysis of KST1, an inward rectifying K+ channel expressed in potato guard cells.
1995,
Pubmed
,
Xenbase
Rich,
Cyclic nucleotide-gated channels colocalize with adenylyl cyclase in regions of restricted cAMP diffusion.
2000,
Pubmed
Root,
Identification of an external divalent cation-binding site in the pore of a cGMP-activated channel.
1993,
Pubmed
,
Xenbase
Rudy,
Families of potassium channel genes in mammals: Toward an understanding of the molecular basis of potassium channel diversity.
1991,
Pubmed
Schachtman,
Expression of an inward-rectifying potassium channel by the Arabidopsis KAT1 cDNA.
1992,
Pubmed
,
Xenbase
Schachtman,
Molecular insights into the structure and function of plant K(+) transport mechanisms.
2000,
Pubmed
Schuurink,
Characterization of a calmodulin-binding transporter from the plasma membrane of barley aleurone.
1998,
Pubmed
Seifert,
Molecular determinants of a Ca2+-binding site in the pore of cyclic nucleotide-gated channels: S5/S6 segments control affinity of intrapore glutamates.
1999,
Pubmed
,
Xenbase
Sentenac,
Cloning and expression in yeast of a plant potassium ion transport system.
1992,
Pubmed
Sunkar,
Expression of a truncated tobacco NtCBP4 channel in transgenic plants and disruption of the homologous Arabidopsis CNGC1 gene confer Pb2+ tolerance.
2000,
Pubmed
Wang,
Dual modulation of renal ATP-sensitive K+ channel by protein kinases A and C.
1991,
Pubmed
Weber,
Ion currents of Xenopus laevis oocytes: state of the art.
1999,
Pubmed
,
Xenbase
White,
The pathways of calcium movement to the xylem.
2001,
Pubmed
Yao,
Primary structure and functional expression of a cGMP-gated potassium channel.
1995,
Pubmed
,
Xenbase
Zagotta,
Structure and function of cyclic nucleotide-gated channels.
1996,
Pubmed
