Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Cell Mol Biol (Noisy-le-grand)
2001 Nov 01;477:1229-39.
Show Gene links
Show Anatomy links
Identification of genes expressed in the Xenopus inner ear.
Serrano EE
,
Trujillo-Provencio C
,
Sultemeier DR
,
Bullock WM
,
Quick QA
.
???displayArticle.abstract???
Recent studies indicate that hearing loss in humans has strong hereditary components associated with expression of specific genes in the auditory apparatus of the inner ear. However, the inner ear poses challenges for molecular research because the amount of tissue that can be isolated is limited, and extraction procedures yield small quantities of RNA and protein. To begin to identity genes essential for auditory function, we synthesized a cDNA library using an RT-PCR protocol and total RNA isolated from eight Xenopus laevis inner ears. Sequence analysis of randomly selected clones demonstrated expression of both identified (calmodulin, SNARE protein, syndecan-2) and unidentified genes, and confirmed synthesis of full length transcripts. Confocal and scanning electron microscopy (SEM) were used to examine the structure of inner ear organs that serve as auditory receptors in amphibians: the sacculus, the amphibian papilla and the basilar papilla. SEM images illustrate the heterogeneity of bundle morphology and demonstrate the continuous appearance of stereociliary bundles in the X. laevis amphibian papilla during larval development and adult life. Investigations of gene expression in Xenopus auditory organs using clones recovered from inner ear cDNA libraries should provide insight regarding the molecular basis of hearing.
Amaya,
A method for generating transgenic frog embryos.
1999, Pubmed,
Xenbase
Amaya,
A method for generating transgenic frog embryos.
1999,
Pubmed
,
Xenbase
Amaya,
Frog genetics: Xenopus tropicalis jumps into the future.
1998,
Pubmed
,
Xenbase
Baird,
Mitotic and nonmitotic hair cell regeneration in the bullfrog vestibular otolith organs.
1996,
Pubmed
Bever,
Ventromedial focus of cell death is absent during development of Xenopus and zebrafish inner ears.
1999,
Pubmed
,
Xenbase
Boatright-Horowitz,
Transient "deafness" accompanies auditory development during metamorphosis from tadpole to frog.
1997,
Pubmed
Brekken,
SPARC, a matricellular protein: at the crossroads of cell-matrix.
2000,
Pubmed
Corwin,
Perpetual production of hair cells and maturational changes in hair cell ultrastructure accompany postembryonic growth in an amphibian ear.
1985,
Pubmed
Corwin,
Identifying the genes of hearing, deafness, and dysequilibrium.
1998,
Pubmed
Díaz,
Quantity, bundle types, and distribution of hair cells in the sacculus of Xenopus laevis during development.
1995,
Pubmed
,
Xenbase
Endege,
Representative cDNA libraries and their utility in gene expression profiling.
1999,
Pubmed
Fettiplace,
Mechanisms of hair cell tuning.
1999,
Pubmed
Friedman,
Unconventional myosins and the genetics of hearing loss.
1999,
Pubmed
Gallagher,
Inductive processes leading to inner ear formation during Xenopus development.
1996,
Pubmed
,
Xenbase
Haddon,
Hyaluronan as a propellant for epithelial movement: the development of semicircular canals in the inner ear of Xenopus.
1991,
Pubmed
,
Xenbase
Hagen,
Assaying the quality of cDNA libraries.
1988,
Pubmed
Hasson,
Unconventional myosins in inner-ear sensory epithelia.
1997,
Pubmed
Heller,
Molecular markers for cell types of the inner ear and candidate genes for hearing disorders.
1998,
Pubmed
Herrler,
Use of SMART-generated cDNA for differential gene expression studies.
2000,
Pubmed
Holme,
Genes involved in deafness.
1999,
Pubmed
Hudspeth,
How hearing happens.
1997,
Pubmed
Hudspeth,
Mechanical amplification of stimuli by hair cells.
1997,
Pubmed
Jahn,
Membrane fusion and exocytosis.
1999,
Pubmed
Kittrell,
The age of diagnosis of sensorineural hearing impairment in children.
1997,
Pubmed
Küssel-Andermann,
Vezatin, a novel transmembrane protein, bridges myosin VIIA to the cadherin-catenins complex.
2000,
Pubmed
Lee,
Prevalence of childhood hearing loss. The Hispanic Health and Nutrition Examination Survey and the National Health and Nutrition Examination Survey II.
1996,
Pubmed
Lefebvre,
Connexins, hearing and deafness: clinical aspects of mutations in the connexin 26 gene.
2000,
Pubmed
Lewis,
Inner ear: dye injection reveals peripheral origins of specific sensitivities.
1982,
Pubmed
López-Anaya,
Development of the Xenopus laevis VIIIth cranial nerve: increase in number and area of axons of the saccular and papillar branches.
1997,
Pubmed
,
Xenbase
Martini,
An introduction to the genetics of normal and defective hearing.
1997,
Pubmed
Mburu,
Mutation analysis of the mouse myosin VIIA deafness gene.
1997,
Pubmed
McGuirt,
Connexin 26 as a cause of hereditary hearing loss.
1999,
Pubmed
Morton,
Genetic epidemiology of hearing impairment.
1991,
Pubmed
Petit,
Genes responsible for human hereditary deafness: symphony of a thousand.
1996,
Pubmed
Shofner,
Quantitative light and scanning electron microscopic study of the developing auditory organs in the bullfrog: implications on their functional characteristics.
1984,
Pubmed
Simmons,
Morphological gradients in sensory hair cells of the amphibian papilla of the frog, Rana pipiens pipiens.
1994,
Pubmed
Skvorak Giersch,
Genetic causes of nonsyndromic hearing loss.
1999,
Pubmed
Smotherman,
Hair cells, hearing and hopping: a field guide to hair cell physiology in the frog.
2000,
Pubmed
Smotherman,
The electrical properties of auditory hair cells in the frog amphibian papilla.
1999,
Pubmed
,
Xenbase
Stone,
Recent insights into regeneration of auditory and vestibular hair cells.
1998,
Pubmed
Teel,
Embryonic expression patterns of Xenopus syndecans.
1996,
Pubmed
,
Xenbase
Trautman,
Developmental expression of syndecan, an integral membrane proteoglycan, correlates with cell differentiation.
1991,
Pubmed
Walker,
Calmodulin controls adaptation of mechanoelectrical transduction by hair cells of the bullfrog's sacculus.
1996,
Pubmed
Whitlon,
A temporospatial map of adhesive molecules in the organ of Corti of the mouse cochlea.
1999,
Pubmed
Xiang,
A modified alkaline lysis miniprep protocol using a single microcentrifuge tube.
1994,
Pubmed
Zimmermann,
A novel isoform of myosin alkali light chain isolated from human muscle cells.
1989,
Pubmed
