XB-ART-7693
J Cell Biol
February 4, 2002;
156
(3):
437-51.
Drosophila Aurora A kinase is required to localize D-TACC to centrosomes and to regulate astral microtubules.
Giet R
,
McLean D
,
Descamps S
,
Lee MJ
,
Raff JW
,
Prigent C
,
Glover DM
.
Abstract
Disruption of the function of the A-type Aurora kinase of Drosophila by mutation or RNAi leads to a reduction in the length of astral microtubules in syncytial embryos, larval neuroblasts, and cultured S2 cells. In neuroblasts, it can also lead to loss of an organized centrosome and its associated aster from one of the spindle poles, whereas the centrosome at the other pole has multiple centrioles. When centrosomes are present at the poles of aurA mutants or aurA RNAi spindles, they retain many antigens but are missing the Drosophila counterpart of mammalian transforming acidic coiled coil (TACC) proteins, D-TACC. We show that a subpopulation of the total Aurora A is present in a complex with D-TACC, which is a substrate for the kinase. We propose that one of the functions of Aurora A kinase is to direct centrosomal organization such that D-TACC complexed to the MSPS/XMAP215 microtubule-associated protein may be recruited, and thus modulate the behavior of astral microtubules.
PubMed ID: 11827981
PMC ID: PMC2173350
Article link: J Cell Biol
Grant support: [+]
Species referenced: Xenopus laevis
Genes referenced: aurka aurkb ckap5 mapt mbp tacc1 tacc3 tbx2 tmprss13 tubg1
Article Images: [+] show captions
References [+] :
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Zhou, Tumour amplified kinase STK15/BTAK induces centrosome amplification, aneuploidy and transformation. 1998, Pubmed
do Carmo Avides, Polo kinase and Asp are needed to promote the mitotic organizing activity of centrosomes. 2001, Pubmed
do Carmo Avides, Abnormal spindle protein, Asp, and the integrity of mitotic centrosomal microtubule organizing centers. 1999, Pubmed
Adams, INCENP binds the Aurora-related kinase AIRK2 and is required to target it to chromosomes, the central spindle and cleavage furrow. 2000, Pubmed , Xenbase
Adams, Essential roles of Drosophila inner centromere protein (INCENP) and aurora B in histone H3 phosphorylation, metaphase chromosome alignment, kinetochore disjunction, and chromosome segregation. 2001, Pubmed
Andersen, Mitotic chromatin regulates phosphorylation of Stathmin/Op18. 1997, Pubmed , Xenbase
Belmont, Catastrophic revelations about Op18/stathmin. 1996, Pubmed
Bischoff, A homologue of Drosophila aurora kinase is oncogenic and amplified in human colorectal cancers. 1998, Pubmed
Blangy, Phosphorylation by p34cdc2 regulates spindle association of human Eg5, a kinesin-related motor essential for bipolar spindle formation in vivo. 1996, Pubmed , Xenbase
Budde, Regulation of Op18 during spindle assembly in Xenopus egg extracts. 2001, Pubmed , Xenbase
Cullen, Msps protein is localized to acentrosomal poles to ensure bipolarity of Drosophila meiotic spindles. 2001, Pubmed
Cullen, mini spindles: A gene encoding a conserved microtubule-associated protein required for the integrity of the mitotic spindle in Drosophila. 1999, Pubmed , Xenbase
Donaldson, Metaphase arrest with centromere separation in polo mutants of Drosophila. 2001, Pubmed
Endow, Spindle dynamics during meiosis in Drosophila oocytes. 1997, Pubmed
Gausz, Genetic characterization of the region between 86F1,2 and 87B15 on chromosome 3 of Drosophila melanogaster. 1982, Pubmed
Gergely, The TACC domain identifies a family of centrosomal proteins that can interact with microtubules. 2001, Pubmed
Gergely, D-TACC: a novel centrosomal protein required for normal spindle function in the early Drosophila embryo. 2000, Pubmed
Giet, Drosophila aurora B kinase is required for histone H3 phosphorylation and condensin recruitment during chromosome condensation and to organize the central spindle during cytokinesis. 2001, Pubmed
Giet, The Xenopus laevis aurora-related protein kinase pEg2 associates with and phosphorylates the kinesin-related protein XlEg5. 1999, Pubmed , Xenbase
Giet, Aurora/Ipl1p-related kinases, a new oncogenic family of mitotic serine-threonine kinases. 1999, Pubmed
Giet, The Xenopus laevis aurora/Ip11p-related kinase pEg2 participates in the stability of the bipolar mitotic spindle. 2000, Pubmed , Xenbase
Glover, Mutations in aurora prevent centrosome separation leading to the formation of monopolar spindles. 1995, Pubmed
Gopalan, A novel mammalian, mitotic spindle-associated kinase is related to yeast and fly chromosome segregation regulators. 1997, Pubmed
Heald, Self-organization of microtubules into bipolar spindles around artificial chromosomes in Xenopus egg extracts. 1996, Pubmed , Xenbase
Heuer, The Drosophila homeotic target gene centrosomin (cnn) encodes a novel centrosomal protein with leucine zippers and maps to a genomic region required for midgut morphogenesis. 1996, Pubmed
Hyman, Morphogenetic properties of microtubules and mitotic spindle assembly. 1996, Pubmed
Kaitna, Incenp and an aurora-like kinase form a complex essential for chromosome segregation and efficient completion of cytokinesis. 2000, Pubmed
Lane, Antibody microinjection reveals an essential role for human polo-like kinase 1 (Plk1) in the functional maturation of mitotic centrosomes. 1997, Pubmed
Lee, Msps/XMAP215 interacts with the centrosomal protein D-TACC to regulate microtubule behaviour. 2001, Pubmed , Xenbase
Megraw, Zygotic development without functional mitotic centrosomes. 2001, Pubmed
Moritz, Microtubule nucleation by gamma-tubulin-containing rings in the centrosome. 1996, Pubmed
Moritz, Recruitment of the gamma-tubulin ring complex to Drosophila salt-stripped centrosome scaffolds. 1998, Pubmed , Xenbase
Ookata, Cyclin B interaction with microtubule-associated protein 4 (MAP4) targets p34cdc2 kinase to microtubules and is a potential regulator of M-phase microtubule dynamics. 1995, Pubmed
Pereira, Centrosome-microtubule nucleation. 1997, Pubmed , Xenbase
Popov, XMAP215 regulates microtubule dynamics through two distinct domains. 2001, Pubmed , Xenbase
Raff, Centrosomes, and not nuclei, initiate pole cell formation in Drosophila embryos. 1989, Pubmed
Rebollo, Visualizing the spindle checkpoint in Drosophila spermatocytes. 2001, Pubmed
Roghi, The Xenopus protein kinase pEg2 associates with the centrosome in a cell cycle-dependent manner, binds to the spindle microtubules and is involved in bipolar mitotic spindle assembly. 1998, Pubmed , Xenbase
Savoian, The rate of poleward chromosome motion is attenuated in Drosophila zw10 and rod mutants. 2001, Pubmed
Sawin, Mitotic spindle organization by a plus-end-directed microtubule motor. 1992, Pubmed , Xenbase
Sawin, Mutations in the kinesin-like protein Eg5 disrupting localization to the mitotic spindle. 1995, Pubmed , Xenbase
Schumacher, A highly conserved centrosomal kinase, AIR-1, is required for accurate cell cycle progression and segregation of developmental factors in Caenorhabditis elegans embryos. 1999, Pubmed
Shirasu, Microtubule dynamics in Xenopus egg extracts. 1999, Pubmed , Xenbase
Speliotes, The survivin-like C. elegans BIR-1 protein acts with the Aurora-like kinase AIR-2 to affect chromosomes and the spindle midzone. 2000, Pubmed
Tournebize, Control of microtubule dynamics by the antagonistic activities of XMAP215 and XKCM1 in Xenopus egg extracts. 2000, Pubmed , Xenbase
Vasquez, XMAP from Xenopus eggs promotes rapid plus end assembly of microtubules and rapid microtubule polymer turnover. 1994, Pubmed , Xenbase
Walter, The mitotic serine/threonine kinase Aurora2/AIK is regulated by phosphorylation and degradation. 2000, Pubmed
Whitfield, The 190 kDa centrosome-associated protein of Drosophila melanogaster contains four zinc finger motifs and binds to specific sites on polytene chromosomes. 1996, Pubmed
Zhou, Tumour amplified kinase STK15/BTAK induces centrosome amplification, aneuploidy and transformation. 1998, Pubmed
do Carmo Avides, Polo kinase and Asp are needed to promote the mitotic organizing activity of centrosomes. 2001, Pubmed
do Carmo Avides, Abnormal spindle protein, Asp, and the integrity of mitotic centrosomal microtubule organizing centers. 1999, Pubmed