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J Cell Biol February 4, 2002; 156 (3): 437-51.

Drosophila Aurora A kinase is required to localize D-TACC to centrosomes and to regulate astral microtubules.

Giet R , McLean D , Descamps S , Lee MJ , Raff JW , Prigent C , Glover DM .

Disruption of the function of the A-type Aurora kinase of Drosophila by mutation or RNAi leads to a reduction in the length of astral microtubules in syncytial embryos, larval neuroblasts, and cultured S2 cells. In neuroblasts, it can also lead to loss of an organized centrosome and its associated aster from one of the spindle poles, whereas the centrosome at the other pole has multiple centrioles. When centrosomes are present at the poles of aurA mutants or aurA RNAi spindles, they retain many antigens but are missing the Drosophila counterpart of mammalian transforming acidic coiled coil (TACC) proteins, D-TACC. We show that a subpopulation of the total Aurora A is present in a complex with D-TACC, which is a substrate for the kinase. We propose that one of the functions of Aurora A kinase is to direct centrosomal organization such that D-TACC complexed to the MSPS/XMAP215 microtubule-associated protein may be recruited, and thus modulate the behavior of astral microtubules.

PubMed ID: 11827981
PMC ID: PMC2173350
Article link: J Cell Biol
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: aurka aurkb ckap5 mapt mbp tacc1 tacc3 tbx2 tmprss13 tubg1

Article Images: [+] show captions
References [+] :
, Protein phosphorylation. Part A. Protein kinases: assays, purification, antibodies, functional analysis, cloning, and expression. 1991, Pubmed