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XB-ART-7843
Genes Dev. January 1, 2002; 16 (1): 58-71.

A novel zinc finger protein is associated with U7 snRNP and interacts with the stem-loop binding protein in the histone pre-mRNP to stimulate 3''-end processing.

Dominski Z , Erkmann JA , Yang X , Sànchez R , Marzluff WF .


Abstract
The stem-loop binding protein (SLBP) is the posttranscriptional regulator of histone mRNA in metazoan cells. SLBP binds histone pre-mRNAs and facilitates 3'-end processing by promoting stable association of U7 snRNP with the pre-mRNA. To identify other factors involved in histone pre-mRNA processing, we used a modified yeast two-hybrid assay in which SLBP and its RNA target were coexpressed as bait. A novel zinc finger protein, hZFP100, which interacts with the SLBP/RNA complex but not with free SLBP, was cloned. The interaction requires regions of SLBP that are important for histone pre-mRNA processing. Antibodies to hZFP100 precipitate U7 snRNA, and expression of hZFP100 in Xenopus oocytes stimulates processing of histone pre-mRNA, showing that hZFP100 is a component of the processing machinery.

PubMed ID: 11782445
PMC ID: PMC155312
Article link: Genes Dev.
Grant support: GM29832 NIGMS NIH HHS

Genes referenced: slbp
Antibodies referenced:

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