XB-ART-7849Nat Cell Biol December 1, 2001; 3 (12): 1086-91.
Distinct AAA-ATPase p97 complexes function in discrete steps of nuclear assembly.
Although nuclear envelope (NE) assembly is known to require the GTPase Ran, the membrane fusion machinery involved is uncharacterized. NE assembly involves formation of a reticular network on chromatin, fusion of this network into a closed NE and subsequent expansion. Here we show that p97, an AAA-ATPase previously implicated in fusion of Golgi and transitional endoplasmic reticulum (ER) membranes together with the adaptor p47, has two discrete functions in NE assembly. Formation of a closed NE requires the p97-Ufd1-Npl4 complex, not previously implicated in membrane fusion. Subsequent NE growth involves a p97-p47 complex. This study provides the first insights into the molecular mechanisms and specificity of fusion events involved in NE formation.
PubMed ID: 11781570
Article link: Nat Cell Biol
Genes referenced: eif4g2 lmnb2 lmnb3 nsfl1c ran ufd1
Antibodies: Lmnb2/3 Ab2
Burke, 2002, Pubmed