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Nat Cell Biol December 1, 2001; 3 (12): 1086-91.

Distinct AAA-ATPase p97 complexes function in discrete steps of nuclear assembly.

Hetzer M , Meyer HH , Walther TC , Bilbao-Cortes D , Warren G , Mattaj IW .

Although nuclear envelope (NE) assembly is known to require the GTPase Ran, the membrane fusion machinery involved is uncharacterized. NE assembly involves formation of a reticular network on chromatin, fusion of this network into a closed NE and subsequent expansion. Here we show that p97, an AAA-ATPase previously implicated in fusion of Golgi and transitional endoplasmic reticulum (ER) membranes together with the adaptor p47, has two discrete functions in NE assembly. Formation of a closed NE requires the p97-Ufd1-Npl4 complex, not previously implicated in membrane fusion. Subsequent NE growth involves a p97-p47 complex. This study provides the first insights into the molecular mechanisms and specificity of fusion events involved in NE formation.

PubMed ID: 11781570
Article link: Nat Cell Biol

Genes referenced: eif4g2 lmnb2 lmnb3 nsfl1c ran ufd1
Antibodies: Lmnb2/3 Ab2

References :
Burke, The nuclear envelope: filling in gaps. 2002, Pubmed

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