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A 250-nucleotide UA-rich element in the 3' untranslated region of Xenopus laevis Vg1 mRNA represses translation both in vivo and in vitro.
Otero LJ
,
Devaux A
,
Standart N
.
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Xenopus laevis Vgl mRNA undergoes both localization and translational control during oogenesis. Vg1 protein does not appear until late stage IV, after localization is complete. To determine whether Vg1 translation is regulated by cytoplasmic polyadenylation, the RACE-PAT method was used. Vg1 mRNA has a constant poly(A) tail throughout oogenesis, precluding a role for cytoplasmic polyadenylation. To identify cis-acting elements involved in Vg1 translational control, the Vg1 3' UTR was inserted downstream of the luciferase ORF and in vitro transcribed, adenylated mRNA injected into stage III or stage VI oocytes. The Vg1 3' UTR repressed luciferase translation in both stages. Deletion analysis of the Vg1 3' UTR revealed that a 250-nt UA-rich fragment, the Vg1 translational element or VTE, which lies 118 nt downstream of the Vg1 localization element, could repress translation as well as the full-length Vg1 3' UTR. Poly(A)-dependent translation is not necessary for repression as nonadenylated mRNAs are also repressed, but cap-dependent translation is required as introduction of the classical swine fever virus IRES upstream of the luciferase coding region prevents repression by the VTE. Repression by the Vg1 3' UTR has been reproduced in Xenopus oocyte in vitro translation extracts, which show a 10-25-fold synergy between the cap and poly(A) tail. A number of proteins UV crosslink to the VTE including FRGY2 and proteins of 36, 42, 45, and 60 kDa. The abundance of p42, p45, and p60 is strikingly higher in stages I-III than in later stages, consistent with a possible role for these proteins in Vg1 translational control.
Asaoka-Taguchi,
Maternal Pumilio acts together with Nanos in germline development in Drosophila embryos.
1999, Pubmed
Asaoka-Taguchi,
Maternal Pumilio acts together with Nanos in germline development in Drosophila embryos.
1999,
Pubmed
Barkoff,
Translational control of cyclin B1 mRNA during meiotic maturation: coordinated repression and cytoplasmic polyadenylation.
2000,
Pubmed
,
Xenbase
Bashirullah,
RNA localization in development.
1998,
Pubmed
,
Xenbase
Bergamini,
Picornavirus IRESes and the poly(A) tail jointly promote cap-independent translation in a mammalian cell-free system.
2000,
Pubmed
Bergsten,
Role for mRNA localization in translational activation but not spatial restriction of nanos RNA.
1999,
Pubmed
Caput,
Identification of a common nucleotide sequence in the 3'-untranslated region of mRNA molecules specifying inflammatory mediators.
1986,
Pubmed
Castagnetti,
Control of oskar mRNA translation by Bruno in a novel cell-free system from Drosophila ovaries.
2000,
Pubmed
Clark,
Synthesis of the posterior determinant Nanos is spatially restricted by a novel cotranslational regulatory mechanism.
2000,
Pubmed
Cote,
A Xenopus protein related to hnRNP I has a role in cytoplasmic RNA localization.
1999,
Pubmed
,
Xenbase
Crucs,
Overlapping but distinct RNA elements control repression and activation of nanos translation.
2000,
Pubmed
Curtis,
Translational regulation in development.
1995,
Pubmed
Dahanukar,
The Nanos gradient in Drosophila embryos is generated by translational regulation.
1996,
Pubmed
Dahanukar,
Smaug, a novel RNA-binding protein that operates a translational switch in Drosophila.
1999,
Pubmed
Dale,
Developmental expression of the protein product of Vg1, a localized maternal mRNA in the frog Xenopus laevis.
1989,
Pubmed
,
Xenbase
Deshler,
A highly conserved RNA-binding protein for cytoplasmic mRNA localization in vertebrates.
1998,
Pubmed
,
Xenbase
Ephrussi,
Oskar organizes the germ plasm and directs localization of the posterior determinant nanos.
1991,
Pubmed
Gautreau,
Two copies of a subelement from the Vg1 RNA localization sequence are sufficient to direct vegetal localization in Xenopus oocytes.
1997,
Pubmed
,
Xenbase
Gavis,
Localization of nanos RNA controls embryonic polarity.
1992,
Pubmed
Gavis,
Translational regulation of nanos by RNA localization.
1994,
Pubmed
Gavis,
A conserved 90 nucleotide element mediates translational repression of nanos RNA.
1996,
Pubmed
Gebauer,
Translational control of dosage compensation in Drosophila by Sex-lethal: cooperative silencing via the 5' and 3' UTRs of msl-2 mRNA is independent of the poly(A) tail.
1999,
Pubmed
Gray,
Control of translation initiation in animals.
1998,
Pubmed
Groisman,
CPEB, maskin, and cyclin B1 mRNA at the mitotic apparatus: implications for local translational control of cell division.
2000,
Pubmed
,
Xenbase
Hake,
CPEB is a specificity factor that mediates cytoplasmic polyadenylation during Xenopus oocyte maturation.
1994,
Pubmed
,
Xenbase
Havin,
RNA-binding protein conserved in both microtubule- and microfilament-based RNA localization.
1998,
Pubmed
,
Xenbase
Hyatt,
The left-right coordinator: the role of Vg1 in organizing left-right axis formation.
1998,
Pubmed
,
Xenbase
Iizuka,
Cap-dependent and cap-independent translation by internal initiation of mRNAs in cell extracts prepared from Saccharomyces cerevisiae.
1994,
Pubmed
Kim-Ha,
oskar mRNA is localized to the posterior pole of the Drosophila oocyte.
1991,
Pubmed
Kim-Ha,
Translational regulation of oskar mRNA by bruno, an ovarian RNA-binding protein, is essential.
1995,
Pubmed
King,
Polarizing genetic information in the egg: RNA localization in the frog oocyte.
1999,
Pubmed
,
Xenbase
Kobayashi,
On the synthesis and destruction of A- and B-type cyclins during oogenesis and meiotic maturation in Xenopus laevis.
1991,
Pubmed
,
Xenbase
Kruys,
Translational blockade imposed by cytokine-derived UA-rich sequences.
1989,
Pubmed
,
Xenbase
Lewis,
Mapping of a minimal AU-rich sequence required for lipopolysaccharide-induced binding of a 55-kDa protein on tumor necrosis factor-alpha mRNA.
1998,
Pubmed
Lie,
Translational regulation of oskar mRNA occurs independent of the cap and poly(A) tail in Drosophila ovarian extracts.
1999,
Pubmed
Macdonald,
The Drosophila pumilio gene: an unusually long transcription unit and an unusual protein.
1992,
Pubmed
Macdonald,
Translational regulation of maternal mRNAs.
1996,
Pubmed
Marinx,
Fertilization of Xenopus eggs imposes a complete translational arrest of mRNAs containing 3'UUAUUUAU elements.
1994,
Pubmed
,
Xenbase
Matthews,
A highly efficient, cell-free translation/translocation system prepared from Xenopus eggs.
1991,
Pubmed
,
Xenbase
Melton,
Translocation of a localized maternal mRNA to the vegetal pole of Xenopus oocytes.
,
Pubmed
,
Xenbase
Mendez,
Phosphorylation of CPEB by Eg2 mediates the recruitment of CPSF into an active cytoplasmic polyadenylation complex.
2000,
Pubmed
,
Xenbase
Mowry,
Complex formation between stage-specific oocyte factors and a Xenopus mRNA localization element.
1996,
Pubmed
,
Xenbase
Mowry,
Vegetal messenger RNA localization directed by a 340-nt RNA sequence element in Xenopus oocytes.
1992,
Pubmed
,
Xenbase
Murata,
Binding of pumilio to maternal hunchback mRNA is required for posterior patterning in Drosophila embryos.
1995,
Pubmed
Murray,
Sequence analysis of cytoplasmic mRNA-binding proteins of Xenopus oocytes identifies a family of RNA-binding proteins.
1992,
Pubmed
,
Xenbase
Pain,
Analysis of translational activity of extracts derived from oocytes and eggs of Xenopus laevis.
1998,
Pubmed
,
Xenbase
Rebagliati,
Identification and cloning of localized maternal RNAs from Xenopus eggs.
1985,
Pubmed
,
Xenbase
Sallés,
Rapid and sensitive analysis of mRNA polyadenylation states by PCR.
1995,
Pubmed
,
Xenbase
Shaw,
A conserved AU sequence from the 3' untranslated region of GM-CSF mRNA mediates selective mRNA degradation.
1986,
Pubmed
Sheets,
The 3'-untranslated regions of c-mos and cyclin mRNAs stimulate translation by regulating cytoplasmic polyadenylation.
1994,
Pubmed
,
Xenbase
Smibert,
smaug protein represses translation of unlocalized nanos mRNA in the Drosophila embryo.
1996,
Pubmed
Smibert,
Smaug, a novel and conserved protein, contributes to repression of nanos mRNA translation in vitro.
1999,
Pubmed
Sonoda,
Recruitment of Nanos to hunchback mRNA by Pumilio.
1999,
Pubmed
St Johnston,
The intracellular localization of messenger RNAs.
1995,
Pubmed
Stebbins-Boaz,
Maskin is a CPEB-associated factor that transiently interacts with elF-4E.
1999,
Pubmed
,
Xenbase
Stebbins-Boaz,
CPEB controls the cytoplasmic polyadenylation of cyclin, Cdk2 and c-mos mRNAs and is necessary for oocyte maturation in Xenopus.
1996,
Pubmed
,
Xenbase
Tannahill,
Localized synthesis of the Vg1 protein during early Xenopus development.
1989,
Pubmed
,
Xenbase
Tautz,
A non-radioactive in situ hybridization method for the localization of specific RNAs in Drosophila embryos reveals translational control of the segmentation gene hunchback.
1989,
Pubmed
Walker,
The clam 3' UTR masking element-binding protein p82 is a member of the CPEB family.
1999,
Pubmed
,
Xenbase
Wang,
Nanos is the localized posterior determinant in Drosophila.
1991,
Pubmed
Weeks,
A maternal mRNA localized to the vegetal hemisphere in Xenopus eggs codes for a growth factor related to TGF-beta.
1987,
Pubmed
,
Xenbase
Wharton,
The Pumilio RNA-binding domain is also a translational regulator.
1998,
Pubmed
Wilhelm,
Coordinate control of translation and localization of Vg1 mRNA in Xenopus oocytes.
2000,
Pubmed
,
Xenbase
Wormington,
Preparation of synthetic mRNAs and analyses of translational efficiency in microinjected Xenopus oocytes.
1991,
Pubmed
,
Xenbase
Xu,
Modulation of the fate of cytoplasmic mRNA by AU-rich elements: key sequence features controlling mRNA deadenylation and decay.
1997,
Pubmed
Yisraeli,
The material mRNA Vg1 is correctly localized following injection into Xenopus oocytes.
1988,
Pubmed
,
Xenbase
de Moor,
Cytoplasmic polyadenylation elements mediate masking and unmasking of cyclin B1 mRNA.
1999,
Pubmed
,
Xenbase
