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Proc Natl Acad Sci U S A January 8, 2002; 99 (1): 257-62.

EDEN-dependent translational repression of maternal mRNAs is conserved between Xenopus and Drosophila.

Ezzeddine N , Paillard L , Capri M , Maniey D , Bassez T , Ait-Ahmed O , Osborne HB .

Translational control is a key level in regulating gene expression in oocytes and eggs because many mRNAs are synthesized and stored during oogenesis for latter use at various stages of oocyte maturation and embryonic development. Understanding the molecular mechanisms that underlie this translational control is therefore crucial. Another important issue is the evolutionary conservation of these mechanisms--in other words the determination of their universal and specific aspects. We report here a comparative analysis of a translational repression mechanism that depends on the EDEN (embryo deadenylation element) element. This small cis-acting element, localized in the 3'' untranslated region of c-mos and Eg mRNAs, was shown to be involved in a deadenylation process. We demonstrate here that in Xenopus embryos, mRNAs that contain an EDEN are translationally repressed. Next, transgenic flies were used to study the effect of the EDEN motif on translation in Drosophila oocytes. We show that this element also causes the translational repression of a reporter gene in Drosophila demonstrating that the EDEN-dependent translational repression is functionally conserved between Xenopus and Drosophila.

PubMed ID: 11756673
PMC ID: PMC117548
Article link: Proc Natl Acad Sci U S A

Species referenced: Xenopus
Genes referenced: mos

References [+] :
Audic, Embryo deadenylation element-dependent deadenylation is enhanced by a cis element containing AUU repeats. 1998, Pubmed, Xenbase