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XB-ART-809
Neuron January 19, 2006; 49 (2): 215-28.

Signaling mechanisms underlying Slit2-induced collapse of Xenopus retinal growth cones.

Piper M , Anderson R , Dwivedy A , Weinl C , van Horck F , Leung KM , Cogill E , Holt C .


Abstract
Slits mediate multiple axon guidance decisions, but the mechanisms underlying the responses of growth cones to these cues remain poorly defined. We show here that collapse induced by Slit2-conditioned medium (Slit2-CM) in Xenopus retinal growth cones requires local protein synthesis (PS) and endocytosis. Slit2-CM elicits rapid activation of translation regulators and MAP kinases in growth cones, and inhibition of MAPKs or disruption of heparan sulfate blocks Slit2-CM-induced PS and repulsion. Interestingly, Slit2-CM causes a fast PS-dependent decrease in cytoskeletal F-actin concomitant with a PS-dependent increase in the actin-depolymerizing protein cofilin. Our findings reveal an unexpected link between Slit2 and cofilin in growth cones and suggest that local translation of actin regulatory proteins contributes to repulsion.

PubMed ID: 16423696
PMC ID: PMC3689199
Article link: Neuron
Grant support: [+]
Genes referenced: actl6a slit2

References [+] :
Abe, Xenopus laevis actin-depolymerizing factor/cofilin: a phosphorylation-regulated protein essential for development. 1996, Pubmed, Xenbase


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