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Model reactions of Cr (VI) with DNA mediated by thiol species.
Krepkiy D
,
Antholine WE
,
Myers C
,
Petering DH
.
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Model reactions were devised to investigate the capacity of physiologically interesting thiol compounds to mediate reactions between CrO4(2-) (Cr (VI)) and DNA. The sulfhydryl containing reagents included cysteine, glutathione, apo-metallothionein (apoMT). Zinc finger 3 of transcription factor IIIA (Zn-F3) of Xenopus laevis was investigated as a potential redox active site of reaction of Cr (VI) and thiol compounds. The DNA samples were calf thymus DNA and two oligomers, one of them specific for binding Zn-F3. Results showed that in the presence of Cr (VI) apoMT readily participated in damaging DNA in a reaction that appeared to be hydroxyl radical dependent. It also became cross-linked to oligomer and native DNA samples. In comparison, the other two thiol donors were largely inactive in these assays even though they, like apoMT, were able to reduce Cr (VI) to Cr (III) under the conditions of the experiments. Direct attempts to cross link thiols with DNA in the presence of Cr3+ were unsuccessful at pH 7.4. Together, the results indicate that apoMT can effectively collaborate with Cr (VI) in reactions that are deleterious to DNA.
Casadevall,
Chromium(VI)-mediated DNA damage: oxidative pathways resulting in the formation of DNA breaks and abasic sites.
1999, Pubmed
Casadevall,
Chromium(VI)-mediated DNA damage: oxidative pathways resulting in the formation of DNA breaks and abasic sites.
1999,
Pubmed
Levina,
In vitro plasmid DNA cleavage by chromium(V) and -(IV) 2-hydroxycarboxylato complexes.
1999,
Pubmed
Liu,
Mutational specificity in a shuttle vector replicating in chromium(VI)-treated mammalian cells.
1999,
Pubmed
Luo,
Role of chromium(IV) in the chromium(VI)-related free radical formation, dG hydroxylation, and DNA damage.
1996,
Pubmed
Mao,
Different conformations and site selectivity of HO-2-Co(III)-bleomycin A2 and Co (III)-bleomycin A2 bound to DNA oligomers.
1996,
Pubmed
Muñoz,
Characterization of the cadmium complex of peptide 49-61: a putative nucleation center for cadmium-induced folding in rabbit liver metallothionein IIA.
1999,
Pubmed
Myers,
Iron stimulates the rate of reduction of hexavalent chromium by human microsomes.
1998,
Pubmed
O'Brien,
Chemical models important in understanding the ways in which chromate can damage DNA.
1994,
Pubmed
Pattanaik,
Basal metallothionein in tumors: widespread presence of apoprotein.
1994,
Pubmed
Petering,
Roles of metallothionein and related proteins in metal metabolism and toxicity: problems and perspectives.
1986,
Pubmed
Sarkar,
Metal replacement in DNA-binding zinc finger proteins and its relevance to mutagenicity and carcinogenicity through free radical generation.
1995,
Pubmed
Shi,
Cr(IV) causes activation of nuclear transcription factor-kappa B, DNA strand breaks and dG hydroxylation via free radical reactions.
1999,
Pubmed
Stearns,
Chromium(VI) reduction by ascorbate: role of reactive intermediates in DNA damage in vitro.
1994,
Pubmed
Sugden,
Direct and hydrogen peroxide-induced chromium(V) oxidation of deoxyribose in single-stranded and double-stranded calf thymus DNA.
1997,
Pubmed
Voitkun,
Cr(III)-mediated crosslinks of glutathione or amino acids to the DNA phosphate backbone are mutagenic in human cells.
1998,
Pubmed
Wiegand,
The reduction of chromium (VI) to chromium (III) by glutathione: an intracellular redox pathway in the metabolism of the carcinogen chromate.
1984,
Pubmed
Zang,
Effects of zinc finger mutations on the nucleic acid binding activities of Xenopus transcription factor IIIA.
1995,
Pubmed
,
Xenbase
Zhitkovich,
Glutathione and free amino acids form stable complexes with DNA following exposure of intact mammalian cells to chromate.
1995,
Pubmed
