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XB-ART-8312
Development October 1, 2001; 128 (19): 3749-58.

Determinants of T box protein specificity.

Conlon FL , Fairclough L , Price BM , Casey ES , Smith JC .


Abstract
Members of the T box family of transcription factors play important roles in early development. Different members of the family exert different effects and here we show that much of the specificity of the Xenopus T box proteins Xbra, VegT and Eomesodermin resides in the DNA-binding domain, or T box. Binding site selection experiments show that the three proteins bind the same core sequence, but they select paired sites that differ in their orientation and spacing. Lysine 149 of Xbra is conserved in all Brachyury homologues, while the corresponding amino acid in VegT and Eomesodermin is asparagine. Mutation of this amino acid to lysine changes the inductive abilities of VegT and Eomesodermin to resemble that of Xbra.

PubMed ID: 11585801
Article link: Development

Genes referenced: eomes tbxt vegt



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