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J Biol Chem. March 24, 2006; 281 (12): 8153-60.

Requirement of the protein B23 for nucleolar disassembly induced by the FRGY2a family proteins.

Gonda K , Wudel J , Nelson D , Katoku-Kikyo N , Reed P , Tamada H , Kikyo N .

In Xenopus somatic cell nuclear cloning, the nucleoli of donor nuclei rapidly and almost completely disappear in egg cytoplasm. We previously showed that the germ cell-specific proteins FRGY2a and FRGY2b were responsible for this unusually drastic nucleolar disassembly. The nucleolar disassembly occurs without inhibition of pre-rRNA transcription, a well known trigger for nucleolar segregation, and the mechanism for the nucleolar disassembly by FRGY2a and FRGY2b remains largely unknown. In this study, we searched for FRGY2a-interacting proteins and investigated the functional consequences of their interactions through a series of experiments. We showed that during the nucleolar disassembly, FRGY2a localized to the nucleoli of isolated nuclei and was capable of disassembling purified nucleoli, suggesting a direct interaction between FRGY2a and nucleolar components. Using a His tag pulldown approach, we identified the abundant and multifunctional nucleolar protein B23 as a potential target of FRGY2a and its related human protein YB1. A specific interaction between FRGY2a/YB1 and B23 was confirmed by co-immunoprecipitation. Finally, B23 knockdown using short interfering RNA and a subsequent add-back experiment confirmed that B23 was necessary for nucleolar disassembly by YB1. We propose that FRGY2a and YB1 disassemble nucleoli by sequestering B23, which is associated with pre-ribosomes and other structurally important nucleolar components.

PubMed ID: 16415342
PMC ID: PMC2222668
Article link: J Biol Chem.
Grant support: R01 GM068027 NIGMS NIH HHS , R01 GM068027-01A1 NIGMS NIH HHS , R01 GM068027-02 NIGMS NIH HHS , R01 GM068027-03 NIGMS NIH HHS

Genes referenced: npm1 ybx1
Antibodies referenced:
Morpholinos referenced:

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