Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Structure of the yeast nucleosome core particle reveals fundamental changes in internucleosome interactions.
White CL
,
Suto RK
,
Luger K
.
???displayArticle.abstract???
Chromatin is composed of nucleosomes, the universally repeating protein-DNA complex in eukaryotic cells. The crystal structure of the nucleosome core particle from Saccharomyces cerevisiae reveals that the structure and function of this fundamental complex is conserved between single-cell organisms and metazoans. Our results show that yeast nucleosomes are likely to be subtly destabilized as compared with nucleosomes from higher eukaryotes, consistent with the idea that much of the yeast genome remains constitutively open during much of its life cycle. Importantly, minor sequence variations lead to dramatic changes in the way in which nucleosomes pack against each other within the crystal lattice. This has important implications for our understanding of the formation of higher order chromatin structure and its modulation by post-translational modifications. Finally, the yeast nucleosome core particle provides a structural context by which to interpret genetic data obtained from yeast. Coordinates have been deposited with the Protein Data Bank under accession number 1ID3.
Aalfs,
What does 'chromatin remodeling' mean?
2000, Pubmed
Aalfs,
What does 'chromatin remodeling' mean?
2000,
Pubmed
Baer,
Eukaryotic RNA polymerase II binds to nucleosome cores from transcribed genes.
1983,
Pubmed
Baxevanis,
A variety of DNA-binding and multimeric proteins contain the histone fold motif.
1995,
Pubmed
Baxevanis,
Histone Sequence Database: new histone fold family members.
1998,
Pubmed
Brünger,
New applications of simulated annealing in X-ray crystallography and solution NMR.
1997,
Pubmed
Carruthers,
The core histone N termini function independently of linker histones during chromatin condensation.
2000,
Pubmed
Esnouf,
Further additions to MolScript version 1.4, including reading and contouring of electron-density maps.
1999,
Pubmed
Finch,
X-ray diffraction study of a new crystal form of the nucleosome core showing higher resolution.
1981,
Pubmed
Glowczewski,
Histone-histone interactions and centromere function.
2000,
Pubmed
Gregory,
Transcription and chromatin converge: lessons from yeast genetics.
2001,
Pubmed
Grunstein,
The regulation of euchromatin and heterochromatin by histones in yeast.
1995,
Pubmed
Grunstein,
Molecular model for telomeric heterochromatin in yeast.
1997,
Pubmed
Grunstein,
Histone acetylation in chromatin structure and transcription.
1997,
Pubmed
Hansen,
Structure and function of the core histone N-termini: more than meets the eye.
1998,
Pubmed
Harp,
Asymmetries in the nucleosome core particle at 2.5 A resolution.
2000,
Pubmed
Hartzog,
Nucleosomes and transcription: recent lessons from genetics.
1997,
Pubmed
Hsu,
Mitotic phosphorylation of histone H3 is governed by Ipl1/aurora kinase and Glc7/PP1 phosphatase in budding yeast and nematodes.
2000,
Pubmed
Hörz,
Deoxyribonuclease II as a probe for chromatin structure. I. Location of cleavage sites.
1980,
Pubmed
Jackson,
Histone H2A.Z has a conserved function that is distinct from that of the major H2A sequence variants.
2000,
Pubmed
Jones,
Improved methods for building protein models in electron density maps and the location of errors in these models.
1991,
Pubmed
Lee,
Structural studies on yeast nucleosomes.
1982,
Pubmed
Luger,
Crystal structure of the nucleosome core particle at 2.8 A resolution.
1997,
Pubmed
Luger,
Characterization of nucleosome core particles containing histone proteins made in bacteria.
1997,
Pubmed
,
Xenbase
Luger,
DNA binding within the nucleosome core.
1998,
Pubmed
Luger,
The histone tails of the nucleosome.
1998,
Pubmed
Luger,
Preparation of nucleosome core particle from recombinant histones.
1999,
Pubmed
,
Xenbase
Luger,
The atomic structure of the nucleosome core particle.
2000,
Pubmed
Moore,
Major role of the histones H3-H4 in the folding of the chromatin fiber.
1997,
Pubmed
Morse,
Yeast nucleosomes allow thermal untwisting of DNA.
1987,
Pubmed
Otwinowski,
Processing of X-ray diffraction data collected in oscillation mode.
1997,
Pubmed
Patterton,
The biochemical and phenotypic characterization of Hho1p, the putative linker histone H1 of Saccharomyces cerevisiae.
1998,
Pubmed
Piñeiro,
Yeast nucleosomal particles: structural and transcriptional properties.
1991,
Pubmed
Robzyk,
Rad6-dependent ubiquitination of histone H2B in yeast.
2000,
Pubmed
Samatey,
Structure of the bacterial flagellar protofilament and implications for a switch for supercoiling.
2001,
Pubmed
Santisteban,
Histone H2A.Z regulats transcription and is partially redundant with nucleosome remodeling complexes.
2000,
Pubmed
Strahl,
Methylation of histone H3 at lysine 4 is highly conserved and correlates with transcriptionally active nuclei in Tetrahymena.
1999,
Pubmed
Strahl,
The language of covalent histone modifications.
2000,
Pubmed
Suto,
Crystal structure of a nucleosome core particle containing the variant histone H2A.Z.
2000,
Pubmed
,
Xenbase
Vogelauer,
Global histone acetylation and deacetylation in yeast.
2000,
Pubmed
Widom,
Structure, dynamics, and function of chromatin in vitro.
1998,
Pubmed
Woodcock,
Higher-order structure of chromatin and chromosomes.
2001,
Pubmed
Workman,
Alteration of nucleosome structure as a mechanism of transcriptional regulation.
1998,
Pubmed
