XB-ART-8511Neurochem Res June 1, 2001; 26 (6): 703-12.
Rapid functional analysis in Xenopus oocytes of Po protein adhesive interactions.
We have developed a coupled Xenopus oocyte expression system for evaluating the functional effects of mutations in known or suspected adhesion molecules, which allows for a very rapid assessment of intercellular adhesion. As a model protein, we first used Protein zero (Po), an adhesion molecule that mediates self-adhesion of the Schwann cell plasma membrane to form compact myelin in the mammalian PNS. A wide variety of mutations in Po cause certain human peripheral neuropathies, such as the Charcot-Marie-Tooth disease (CMT) type 1B and Dejerine-Sottas syndrome (DSS). After wild-type Po mRNA is injected, the protein is synthesized and correctly targeted to the oocyte cell surface. When two oocytes are paired, wild-type Po redistributes and concentrates at the cell-cell apposition region, and by electron microscopy, the oocyte pairs show close cell-cell appositions and are devoid of the microvilli that are observed in uninjected oocyte pairs. These are hallmark features of highly adhesive cell:cell interfaces. Several point mutations in Po were engineered, corresponding to the molecular defects in the CMT type 1B or DSS. The proteins encoded by these mutations reached the cell surface but failed to concentrate at the oocyte interface. Po carrying a point mutation that is found in DSS is not targeted on the plasma membrane and fail to accumulate at the cell-cell contact site.
PubMed ID: 11519730
Article link: Neurochem Res
Species referenced: Xenopus laevis
Genes referenced: egr2 mpz pmp22 prx
GO keywords: apical junction complex
Disease Ontology terms: Charcot-Marie-Tooth disease type 1B
OMIMs: CHARCOT-MARIE-TOOTH DISEASE, DEMYELINATING, TYPE 1B; CMT1B
References [+] :
Brackenbury, Adhesion among neural cells of the chick embryo. I. An immunological assay for molecules involved in cell-cell binding. 1977, Pubmed