Due to necessary maintenance, Xenbase will be unavailable December 24-30, 2014. We apologize for the inconvenience.

Click on this message to dismiss it.
Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
Cell. June 1, 2001; 105 (5): 645-55.

Emi1 is a mitotic regulator that interacts with Cdc20 and inhibits the anaphase promoting complex.

Reimann JD , Freed E , Hsu JY , Kramer ER , Peters JM , Jackson PK .

We have discovered an early mitotic inhibitor, Emi1, which regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). Emi1 is a conserved F box protein containing a zinc binding region essential for APC inhibition. Emi1 accumulates before mitosis and is ubiquitylated and destroyed in mitosis, independent of the APC. Emi1 immunodepletion from cycling Xenopus extracts strongly delays cyclin B accumulation and mitotic entry, whereas nondestructible Emi1 stabilizes APC substrates and causes a mitotic block. Emi1 binds the APC activator Cdc20, and Cdc20 can rescue an Emi1-induced block to cyclin B destruction. Our results suggest that Emi1 regulates progression through early mitosis by preventing premature APC activation, and may help explain the well-known delay between cyclin B/Cdc2 activation and cyclin B destruction.

PubMed ID: 11389834
Article link: Cell.
Grant support: CA09302 NCI NIH HHS , CA09302 NCI NIH HHS , CA09302 NCI NIH HHS , CA09302 NCI NIH HHS , CA09302 NCI NIH HHS , CA09302 NCI NIH HHS , GM07365 NIGMS NIH HHS , GM54811 NIGMS NIH HHS , GM60439 NIGMS NIH HHS

Genes referenced: ccnb1.2 cdc20 cdk1 fbxo5 pold1
Antibodies referenced:
Morpholinos referenced:

My Xenbase: [ Log-in / Register ]
version: [3.3.1]

Major funding for Xenbase is provided by the National Institute of Child Health and Human Development, grant P41 HD064556