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Dev Dyn May 1, 2001; 221 (1): 14-25.

A novel POZ/zinc finger protein, champignon, interferes with gastrulation movements in Xenopus.

Goto T , Hasegawa K , Kinoshita T , Kubota HY .

We have cloned a novel krüppel-like transcription factor of Xenopus that encodes POZ/zinc finger protein by expression cloning. Overexpression of mRNA resulted in interference with gastrulation. Because the injected embryo looks like a mushroom in appearance at the neurula stage, we have named this gene champignon (cpg). In cpg-injected embryos, the blastopore appeared normally, but regressed thereafter. The injected embryos then elongated along the primary dorsoventral axis during the tailbud stage. Histologic sections and reverse transcription-polymerase chain reaction analysis showed that cpg had no effect on the cell differentiation. The animal pole region of cpg-injected embryos was thick during the gastrula stage, and mesodermal cells remained in the marginal zone. Furthermore, neither Keller-sandwich explants nor activin-treated animal cap explants excised from cpg-injected embryos elongated. These results suggest that cpg acts as a potent inhibitor of cell migration and cell intercalation during gastrulation.

PubMed ID: 11357190
Article link: Dev Dyn

Genes referenced: bgn cdh3 col1a1 fn1 gata2 gsc h4c1 mal myod1 ncam1 nog otx2 pcdh8.2 sia1 tbxt wipf2 wnt8a zbtb7b

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