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Dev Biol. September 1, 2006; 297 (1): 103-17.

FGF is essential for both condensation and mesenchymal-epithelial transition stages of pronephric kidney tubule development.

Urban AE , Zhou X , Ungos JM , Raible DW , Altmann CR , Vize PD .

The pronephros is a transient embryonic kidney that is essential for the survival of aquatic larvae. It is also absolutely critical for adult kidney development, as the pronephric derivative the wolffian duct forms the ductal system of the adult kidney and also triggers the condensation of metanephric mesenchyme into the adult nephrons. While exploring Xenopus pronephric patterning, we observed that epidermally delivered hedgehog completely suppresses pronephric kidney tubule development but does not effect development of the pronephric glomus, the equivalent of the mammalian glomerulus or corpuscle. This effect is not mediated by apoptosis. Microarray analysis of microdissected primordia identified FGF8 as one of the potential mediators of hedgehog action. Further investigation demonstrated that SU5402-sensitive FGF signaling plays a critical role in the very earliest stages of pronephric tubule development. Modulation of FGF8 activity using a morpholino has a later effect that blocks condensation of pronephric mesenchyme into the pronephric tubule. Together, these data show that FGF signaling plays a critical role at two stages of embryonic kidney development, one in the condensation of the pronephric primordium from the intermediate mesoderm and a second in the later epithelialization of this mesenchyme into the pronephric nephron. The data also show that in Xenopus, development of the glomus/glomerulus can be uncoupled from nephron formation via ectopic hedgehog expression and provides an experimental avenue for investigating glomerulogenesis in the complete absence of tubules.

PubMed ID: 16872594
Article link: Dev Biol.
Grant support: T32 HD007183 NICHD NIH HHS

Genes referenced: aldh1a1 arnt2 bmp2 ccdc42 cyp17a1 dkk1 egr1 eno3 esr10 fgf8 fn1 foxd1 foxd2 foxf1 frzb2 fst gata5 grem1 hand1 hhip hnf4a ihh lhx1 myf5 myod1 nodal1 nphs1 osr2 pard6b pax2 pax3 pax8 ptch2 shh slc12a1 slc5a8 snai1 twist1 wif1 wnt4 wnt8a wt1

Antibodies referenced: Somite Ab1
Morpholinos referenced: fgf8 MO3

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