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The Y14 protein communicates to the cytoplasm the position of exon-exon junctions.
Kim VN
,
Yong J
,
Kataoka N
,
Abel L
,
Diem MD
,
Dreyfuss G
.
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We recently described an RNA-binding protein, Y14, that binds preferentially to spliced mRNAs and persists in the cytoplasm. Y14 is part of a multi-protein complex that also contains the mRNA export factor TAP. This suggests that splicing imprints the mRNA with a unique set of proteins that communicate the history of the transcript to the cytoplasm. Here, using microinjection of pre-mRNAs into Xenopus oocyte nuclei followed by immunoprecipitation of RNase-fragmented mRNAs from the cytoplasm, we show that Y14 is stably bound to sequences immediately upstream of exon-exon junctions. This feature appears to be unique to Y14. Using monoclonal antibodies that we produced against Aly/REF, another component recently reported to be an mRNA export factor, we show that Aly/REF is associated with spliced mRNAs in the nucleus but is not detectable on mRNAs in the cytoplasm. Thus, we propose that the splicing- dependent binding of Y14 provides a position-specific molecular memory that communicates to the cytoplasm the location of exon and intron boundaries. This novel mechanism may play an important role in post-splicing events.
Blencowe,
A coactivator of pre-mRNA splicing.
1998, Pubmed
Blencowe,
A coactivator of pre-mRNA splicing.
1998,
Pubmed
Bochnig,
A monoclonal antibody against 2,2,7-trimethylguanosine that reacts with intact, class U, small nuclear ribonucleoproteins as well as with 7-methylguanosine-capped RNAs.
1987,
Pubmed
Cheng,
Translation to near the distal end of the penultimate exon is required for normal levels of spliced triosephosphate isomerase mRNA.
1990,
Pubmed
Cheng,
Introns are cis effectors of the nonsense-codon-mediated reduction in nuclear mRNA abundance.
1994,
Pubmed
Culbertson,
RNA surveillance. Unforeseen consequences for gene expression, inherited genetic disorders and cancer.
1999,
Pubmed
Czaplinski,
The surveillance complex interacts with the translation release factors to enhance termination and degrade aberrant mRNAs.
1998,
Pubmed
Czaplinski,
Should we kill the messenger? The role of the surveillance complex in translation termination and mRNA turnover.
1999,
Pubmed
Frischmeyer,
Nonsense-mediated mRNA decay in health and disease.
1999,
Pubmed
Hentze,
A perfect message: RNA surveillance and nonsense-mediated decay.
1999,
Pubmed
Hilleren,
mRNA surveillance in eukaryotes: kinetic proofreading of proper translation termination as assessed by mRNP domain organization?
1999,
Pubmed
Izaurralde,
Transport of macromolecules between the nucleus and the cytoplasm.
1998,
Pubmed
Kataoka,
Pre-mRNA splicing imprints mRNA in the nucleus with a novel RNA-binding protein that persists in the cytoplasm.
2000,
Pubmed
,
Xenbase
Le Hir,
Pre-mRNA splicing alters mRNP composition: evidence for stable association of proteins at exon-exon junctions.
2000,
Pubmed
Le Hir,
The spliceosome deposits multiple proteins 20-24 nucleotides upstream of mRNA exon-exon junctions.
2000,
Pubmed
,
Xenbase
Li,
Nonsense surveillance in lymphocytes?
1998,
Pubmed
Luo,
Splicing is required for rapid and efficient mRNA export in metazoans.
1999,
Pubmed
,
Xenbase
Matsumoto,
Nuclear history of a pre-mRNA determines the translational activity of cytoplasmic mRNA.
1998,
Pubmed
,
Xenbase
Mayeda,
Purification and characterization of human RNPS1: a general activator of pre-mRNA splicing.
1999,
Pubmed
McGarvey,
The acute myeloid leukemia-associated protein, DEK, forms a splicing-dependent interaction with exon-product complexes.
2000,
Pubmed
Muhlrad,
Premature translational termination triggers mRNA decapping.
1994,
Pubmed
Nagy,
A rule for termination-codon position within intron-containing genes: when nonsense affects RNA abundance.
1998,
Pubmed
Piñol-Roma,
Shuttling of pre-mRNA binding proteins between nucleus and cytoplasm.
1992,
Pubmed
,
Xenbase
Shyu,
The double lives of shuttling mRNA binding proteins.
2000,
Pubmed
Siomi,
Transportin-mediated nuclear import of heterogeneous nuclear RNP proteins.
1997,
Pubmed
Stutz,
REF, an evolutionary conserved family of hnRNP-like proteins, interacts with TAP/Mex67p and participates in mRNA nuclear export.
2000,
Pubmed
,
Xenbase
Thermann,
Binary specification of nonsense codons by splicing and cytoplasmic translation.
1998,
Pubmed
Watakabe,
A secondary structure at the 3' splice site affects the in vitro splicing reaction of mouse immunoglobulin mu chain pre-mRNAs.
1989,
Pubmed
Zhang,
Polysome-associated mRNAs are substrates for the nonsense-mediated mRNA decay pathway in Saccharomyces cerevisiae.
1997,
Pubmed
Zhang,
At least one intron is required for the nonsense-mediated decay of triosephosphate isomerase mRNA: a possible link between nuclear splicing and cytoplasmic translation.
1998,
Pubmed
Zhou,
The protein Aly links pre-messenger-RNA splicing to nuclear export in metazoans.
2000,
Pubmed
