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XB-ART-9222
EMBO J. April 17, 2001; 20 (8): 2062-8.

The Y14 protein communicates to the cytoplasm the position of exon-exon junctions.

Kim VN , Yong J , Kataoka N , Abel L , Diem MD , Dreyfuss G .


Abstract
We recently described an RNA-binding protein, Y14, that binds preferentially to spliced mRNAs and persists in the cytoplasm. Y14 is part of a multi-protein complex that also contains the mRNA export factor TAP. This suggests that splicing imprints the mRNA with a unique set of proteins that communicate the history of the transcript to the cytoplasm. Here, using microinjection of pre-mRNAs into Xenopus oocyte nuclei followed by immunoprecipitation of RNase-fragmented mRNAs from the cytoplasm, we show that Y14 is stably bound to sequences immediately upstream of exon-exon junctions. This feature appears to be unique to Y14. Using monoclonal antibodies that we produced against Aly/REF, another component recently reported to be an mRNA export factor, we show that Aly/REF is associated with spliced mRNAs in the nucleus but is not detectable on mRNAs in the cytoplasm. Thus, we propose that the splicing- dependent binding of Y14 provides a position-specific molecular memory that communicates to the cytoplasm the location of exon and intron boundaries. This novel mechanism may play an important role in post-splicing events.

PubMed ID: 11296238
PMC ID: PMC125236
Article link: EMBO J.

Genes referenced: alyref rbm8a
Antibodies referenced:

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