Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
XB-ART-9596
FEBS Lett 2001 Feb 09;4901-2:49-53. doi: 10.1016/s0014-5793(01)02136-6.
Show Gene links Show Anatomy links

Multiple PIP2 binding sites in Kir2.1 inwardly rectifying potassium channels.

Soom M , Schönherr R , Kubo Y , Kirsch C , Klinger R , Heinemann SH .


???displayArticle.abstract???
Inwardly rectifying potassium channels require binding of phosphatidylinositol-4,5-bisphosphate (PIP2) for channel activity. Three independent sites (aa 175-206, aa 207-246, aa 324-365) were located in the C-terminal domain of Kir2.1 channels by assaying the binding of overlapping fragments to PIP2 containing liposomes. Mutations in the first site, which abolished channel activity, reduced PIP2 binding of this fragment but not of the complete C-terminus. Point mutations in the third site also reduced both, channel activity and PIP2 binding of this segment. The relevance of the third PIP2 binding site provides a basis for the understanding of constitutively active Kir2 channels.

???displayArticle.pubmedLink??? 11172809
???displayArticle.link??? FEBS Lett


Species referenced: Xenopus laevis
Genes referenced: kcnj2