Due to necessary maintenance, Xenbase will be unavailable December 24-30, 2014. We apologize for the inconvenience.

Click on this message to dismiss it.
Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
XB-ART-9806
Science. December 22, 2000; 290 (5500): 2309-12.

Inhibition of eukaryotic DNA replication by geminin binding to Cdt1.

Wohlschlegel JA , Dwyer BT , Dhar SK , Cvetic C , Walter JC , Dutta A .


Abstract
In all eukaryotic organisms, inappropriate firing of replication origins during the G2 phase of the cell cycle is suppressed by cyclin-dependent kinases. Multicellular eukaryotes contain a second putative inhibitor of re-replication called geminin. Geminin is believed to block binding of the mini-chromosome maintenance (MCM) complex to origins of replication, but the mechanism of this inhibition is unclear. Here we show that geminin interacts tightly with Cdt1, a recently identified replication initiation factor necessary for MCM loading. The inhibition of DNA replication by geminin that is observed in cell-free DNA replication extracts is reversed by the addition of excess Cdt1. In the normal cell cycle, Cdt1 is present only in G1 and S, whereas geminin is present in S and G2 phases of the cell cycle. Together, these results suggest that geminin inhibits inappropriate origin firing by targeting Cdt1.

PubMed ID: 11125146
Article link: Science.
Grant support: CA60499 NCI NIH HHS , CA60499 NCI NIH HHS , CA60499 NCI NIH HHS , CA60499 NCI NIH HHS , CA60499 NCI NIH HHS , CA60499 NCI NIH HHS

Genes referenced: cdt1 gmnn
Antibodies referenced:
Morpholinos referenced:

My Xenbase: [ Log-in / Register ]
version: [3.3.1]


Major funding for Xenbase is provided by the National Institute of Child Health and Human Development, grant P41 HD064556