Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
XB-ART-9868
Biophys J 2000 Dec 01;796:3294-306.
Show Gene links Show Anatomy links

Fluorescence correlation spectroscopy in small cytosolic compartments depends critically on the diffusion model used.

Gennerich A , Schild D .


???displayArticle.abstract???
Fluorescence correlation spectroscopy (FCS) is a powerful technique for measuring low concentrations of fluorescent molecules and their diffusion constants. In the standard case, fluorescence fluctuations are measured in an open detection volume defined by the confocal optics. However, if FCS measurements are carried out in cellular processes that confine the detection volume, the standard FCS model leads to erroneous results. In this paper, we derive a modified FCS model that takes into account the confinement of the detection volume. Using this model, we have carried out the first FCS measurements in dendrites of cultured neurons. We further derive, for the case of confined diffusion, the limits within which the standard two- and three-dimensional diffusion models give reliable results.

???displayArticle.pubmedLink??? 11106632
???displayArticle.pmcLink??? PMC1301203
???displayArticle.link??? Biophys J



References [+] :
Berland, Two-photon fluorescence correlation spectroscopy: method and application to the intracellular environment. 1995, Pubmed