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XB-ART-9962
Cell. October 27, 2000; 103 (3): 435-47.

CPEB, maskin, and cyclin B1 mRNA at the mitotic apparatus: implications for local translational control of cell division.

Groisman I , Huang YS , Mendez R , Cao Q , Theurkauf W , Richter JD .


Abstract
In Xenopus development, the expression of several maternal mRNAs is regulated by cytoplasmic polyadenylation. CPEB and maskin, two factors that control polyadenylation-induced translation are present on the mitotic apparatus of animal pole blastomeres in embryos. Cyclin B1 protein and mRNA, whose translation is regulated by polyadenylation, are colocalized with CPEB and maskin. CPEB interacts with microtubules and is involved in the localization of cyclin B1 mRNA to the mitotic apparatus. Agents that disrupt polyadenylation-induced translation inhibit cell division and promote spindle and centrosome defects in injected embryos. Two of these agents inhibit the synthesis of cyclin B1 protein and one, which has little effect on this process, disrupts the localization of cyclin B1 mRNA and protein. These data suggest that CPEB-regulated mRNA translation is important for the integrity of the mitotic apparatus and for cell division.

PubMed ID: 11081630
Article link: Cell.
Grant support: GM46779 NIGMS NIH HHS , GM50898 NIGMS NIH HHS

Genes referenced: bub3 ccnb1 cpeb1 eif4e elavl1 mapk1 tacc3
Antibodies referenced:
Morpholinos referenced:
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