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Dev Biol November 1, 2000; 227 (1): 183-96.

Ras-mediated FGF signaling is required for the formation of posterior but not anterior neural tissue in Xenopus laevis.

Ribisi S , Mariani FV , Aamar E , Lamb TM , Frank D , Harland RM .

Fibroblast growth factor (FGF) has been proposed to be involved in the specification and patterning of the developing vertebrate nervous system. There is conflicting evidence, however, concerning the requirement for FGF signaling in these processes. To provide insight into the signaling mechanisms that are important for neural induction and anterior-posterior neural patterning, we have employed the dominant negative Ras mutant, N17Ras, in addition to a truncated FGF receptor (XFD). Both N17Ras and XFD, when expressed in Xenopus laevis animal cap ectoderm, inhibit the ability of FGF to generate neural pattern. They also block induction of posterior neural tissue by XBF2 and XMeis3. However, neither XFD nor N17Ras inhibits noggin, neurogenin, or XBF2 induction of anterior neural markers. MAP kinase activation has been proposed to be necessary for neural induction, yet N17Ras inhibits the phosphorylation of MAP kinase that usually follows explantation of explants. In whole embryos, Ras-mediated FGF signaling is critical for the formation of posterior neural tissues but is dispensable for neural induction.

PubMed ID: 11076686
Article link: Dev Biol

Species referenced: Xenopus laevis
Genes referenced: acta4 actc1 actl6a dusp6 eef1a2 egr2 en2 fgf2 foxa1 foxd1 hoxb9 mapk1 meis3 myod1 ncam1 neurog1 nog nrp1 otx2 sox2

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