Methods & Materials: techniques for Xenopus research - Xenbase | A Xenopus laevis and Xenopus tropicalis resource

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Gurdon wins Nobel!
Xenopus laevis genome ^New!
15th International Xenopus Meeting
August 24th - 28th, 2014
Asilomar, CA ^New!
17th International Congress of Developmental Biology
June 16-20, 2013 Cancun, Mexico
MBL Bioinformatics Workshop
Next Workshop, May 2014
Xenopus PAR grant due September 30, 2013
Developmental Neurobiology: Special Xenopus Issue
Final version of the 2011 Xenopus Community White Paper posted
Xenopus tropicalis genome draft 7.1 viewable in the Xenbase Genome Browser

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JGI Xentr7.1 models viewable in Xenbase gene pages, gbrowse, and blast

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X tropicalis
JGI (sequence)
trops @ U.Virginia
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ZFIN(Zebrafish)
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FlyBase(Drosophila)
WormBase(Nematode)
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Methods and materials: techniques for Xenopus research

Introduction to Xenopus

Animals Xenopus laevis and tropicalis	hCG stimulation (Harland lab) isolating testes (Harland lab) squeezing (Harland lab) IVF (Harland lab) laevis developmental timetable (Harland lab) tropicalis developmental timetable (Harland lab)	Embryological Methods Methods for manipulating and analysing development	oocyte transfer technique dejelly (Harland lab) microinject (Harland lab) animal caps using gastromaster (5 mb mpg) organizer transplant via gastromaster (8 mb mpg)

Reagents and Materials Everything you need to work with frogs	cDNA libraries frogs morpholinos plasmids/phage RT-PCR primers delivery service of oocytes	Digital Photography Information on cameras, software, etc.	Qimaging mediacybernetics

Protocols	making affinity columns running affinity columns antisense oligos fluorescent in situs and FCIS in situ hybridization (Harland lab) oocyte transfer TUNEL staining	Frog Research Equipment	holding systems and housing equipments

Xenopus stage development time / temperature charts