Apoptosis patterns in developing
Xenopus embryos detected via TUNEL
These images of programmed
cell death patterns analyzed by whole-mount in situ DNA end-labeling technique
(TUNEL: TdT-mediated dUTP digoxigenin nick end labeling) were submitted by Jean
Gautier.
For a detailed descriptions
of the images please see: Hensey C. and Gautier J. (1998). Program cell death
during Xenopus development: a spatio-temporal analysis. Dev.
Biol. 203, 36-48. TUNEL protocols and additional references are also available
below.
This page contains previously
published text and images. Copyright © to all such materials belongs to
Academic Press (1998).
Click
on images to view enlargements (6K to 7K, < 2 seconds).
| Method |
Original
description(s): |
| TUNEL |
Hensey C.
and Gautier J. (1998). Program cell death during Xenopus development:
a spatio-temporal analysis. Dev.
Biol. 203, 36-48.
Veenstra,
GJ, Peterson-Maduro J, Mathu MT, van der Vliet PC, Destree OHJ. (1998).
Non-cell autonomous induction of apoptosis and loss of posterior structures
by activation domain-specific interactions of Oct-1 in the Xenopus embryo.
Cell
Death Differ 5:774-84.
|
| Images
submitted by: |
Protocol
Specific Reagents/Suppliers |
| Jean
Gautier |
|
| Protocol:
(submitted by Pengfei Lu and Peter Vize) |
Printer
friendly version of protocol |
| soon |
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Please
reference this protocol version as:
Lu,
P. and Vize, P.D. (2001). Xenbase protocols document #XBP00001. http://xenbase.org
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Links
to other on-line TUNEL protocols |
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