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Apoptosis patterns in developing Xenopus embryos detected via TUNEL

These images of programmed cell death patterns analyzed by whole-mount in situ DNA end-labeling technique (TUNEL: TdT-mediated dUTP digoxigenin nick end labeling) were submitted by Jean Gautier.

For a detailed descriptions of the images please see: Hensey C. and Gautier J. (1998). Program cell death during Xenopus development: a spatio-temporal analysis. Dev. Biol. 203, 36-48. TUNEL protocols and additional references are also available below.

This page contains previously published text and images. Copyright © to all such materials belongs to Academic Press (1998).
Click on images to view enlargements (6K to 7K, < 2 seconds).

Stg 10.5 vegetal view Stg 11.5 vegetal view Stg 12.5 animal view Stg 13 dorsal view
Stage 10.5, vegetal view stage 11.5, vegetal view stage 12.5, animal view
stage 13 dorsal view
     
Stg 15 anterior view Stg 17 dorsal view Stg 18 dorsal view Stg 23 dorsal view
stage 15 stage 17 stage 18 stage 23
Stg 32 dorsal view
stage 32

 

Method Original description(s):
TUNEL

Hensey C. and Gautier J. (1998). Program cell death during Xenopus development: a spatio-temporal analysis. Dev. Biol. 203, 36-48.

Veenstra, GJ, Peterson-Maduro J, Mathu MT, van der Vliet PC, Destree OHJ. (1998). Non-cell autonomous induction of apoptosis and loss of posterior structures by activation domain-specific interactions of Oct-1 in the Xenopus embryo. Cell Death Differ 5:774-84.
Images submitted by: Protocol Specific Reagents/Suppliers
Jean Gautier  
Protocol: (submitted by Pengfei Lu and Peter Vize) Printer friendly version of protocol
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Please reference this protocol version as:

Lu, P. and Vize, P.D. (2001). Xenbase protocols document #XBP00001. http://xenbase.org

 Links to other on-line TUNEL protocols

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