Apoptosis patterns in developing Xenopus embryos detected via TUNELThese images of programmed cell death patterns analyzed by whole-mount in situ DNA end-labeling technique (TUNEL: TdT-mediated dUTP digoxigenin nick end labeling) were submitted by Jean Gautier.
For a detailed descriptions of the images please see: Hensey C. and Gautier J. (1998). Program cell death during Xenopus development: a spatio-temporal analysis. Dev. Biol. 203, 36-48. TUNEL protocols and additional references are also available below.
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|Stage 10.5, vegetal view||stage 11.5, vegetal view||stage 12.5, animal view||stage 13 dorsal view|
|stage 15||stage 17||stage 18||stage 23|
Hensey C. and Gautier J. (1998). Program cell death during Xenopus development: a spatio-temporal analysis. Dev. Biol. 203, 36-48.
Veenstra, GJ, Peterson-Maduro J, Mathu MT, van der Vliet PC, Destree OHJ. (1998). Non-cell autonomous induction of apoptosis and loss of posterior structures by activation domain-specific interactions of Oct-1 in the Xenopus embryo. Cell Death Differ 5:774-84.
|Images submitted by:||Protocol Specific Reagents/Suppliers|
|Protocol: (submitted by Pengfei Lu and Peter Vize)||Printer friendly version of protocol|
Please reference this protocol version as:
Lu, P. and Vize, P.D. (2001). Xenbase protocols document #XBP00001. http://xenbase.org
|Links to other on-line TUNEL protocols|