Methods/Materials : RT-PCR primers

• XASH-3a,b (Zimmerman et al., 1993; Turner and Weintraub, 1994), (219 bp and A224 bp)
  5'-GGACTCTCGCCTTGTGGC-3'(470-487)
  5'-GATATGTTCTTGTAATAGTCAGT-3'(469-486)
• XATH-3 (Takebayashi et al., 1997), (250 bp)
  5'-TGGACCTCAGGCCATGTTC-3'(501-519)
  5'-GATGCTGAGTGGAGGTGTTA-3'(731-750)
• Cardiac actin:
  U: 5-TCC CTG TAC GCT TCT GGT CGT A-3
  D: 5-TCT CAA AGT CCA AAG CCA CAT A-3
• chordin (Sasai et al., 1994), (211 bp)
  5'-AACTGCCAGGACTGGATGGT-3'(3190-3209)
  5'-GGCAGGATTTAGAGTTGCTTC-3'(3380-3400)
• CG13:
  U 5-AGT TGT TGA TTT TGT GAA AAC C-3
  D 5-CTT CTT CTA AAT CAA CAA CAG G-3
• EF1alpha: PCR product 268 bp
  U: 5-CAG ATT GGT GCT GGA TAT GC-3 (1104-1123)
  D: 5'-ACT GCC TTG ATG ACT CCT AG-3' (1372-1353)
• En2: PCR product; 305 bp
  U: 5-CGG AAT TCA TCA GGT CCG AGA TC-3 (474-490)
  D: 5-GCG GAT CCT TTG AAG TGG TCG CG-3 (779-759)
• Epidermal keratin:
  U 5-CAC CAG AAC ACA GAG TAC-3
  D 5-CAA CCT TCC CAT CAA CCA-3
• F-Spondin:
  U 5-TCT GGC AGT ATG TGG CAA CGT C-3
  D 5-GTA CAA TGC TCG CCT TGA GTC TC-3
• geminin (early neural)
  U 5-GCTGGACATGTACAGTACA-3' (bp 505-523)
  D 5-TCACCTCACATAAAGGCTGG-3' (bp 964-983)
  
• Goosecoid; 278 bp
  U: 5-ACA ACT GGA AGC ACT GGA-3 (624-641)
  D: 5-TCT TAT TCC AGA GGA ACC-3 (902-885)
• GS17:
  U: 5-ATG CCA GTC CAA CTT CAA GGC A-3
  D: 5-CCT CTA GCA TAG ATG GAC TGT A-3
• Krox 20: PCR product 448 bp
  U: 5-AAC CGC CCC AGT AAG ACC-3 (935-951)
  D: 5-GTG TCA GCC TGT CCT GTT AG-3 (1383 1364)
• Mix1: PCR product 400 bp.
  U: 5-AAT GTC TCA AGG CAG AGG-3 (741-758)
  D: 5-GTG TCA CTG ACA CCA GAA-3 (1141-1124)
• Muscle actin: PCR product 222 bp.
  U: 5-GCT GAC AGA ATG CAG AAG-3 (992-1010)
  D. 5-TTG CTT GGA GGA GTG TGT-3 (1214-1196)
• NCAM: PCR product 342 bp
  U: 5-CAC AGT TCC ACC AAA TGC-3 (2817-2835)
  D. 5-GGA ATC AAG CGG TAC AGA-3 (3142-3159)
  
• NeuroD (Lee et al., 1995), (238 bp)
  5'-GTGAAATCCCAATAGACACC-3'(975-994)
  5'-TTCCCCATATCTAAAGGCAG-3'(1212-1193)
• NF-M:
  F, 5'-GAA CAG TAC GCC AAG CTG ACT G-3
  R, 5'-GCA GCA ATT TCT ATA TCC AGA G-3'
• X-ngnr-1a (Ma et al., 1996),(334 bp)
  5'-CAAGAGCGGAGAAACTGTGT-3'(563-582)
  5'-GAAGGAGCAACAAGAGGAAG-3'(896-877)
• Noggin: PCR product 277 bp
  U: 5-AGT TGC AGA TGT GGC TCT-3 (404-421)
  D: 5-AGT CCA AGA GTC TGA GCA-3 (681-665)
• N-tubulin (Good et al., 1989), (250 bp)
  5'-ACACGGCATTGATCCTACAG-3'(169-188)
  5'-AGCTCCTTCGGTGTAATGAC-3'(418-399)
• o ODC: PCR product 385 bp
  U: 5'-GTC AAT GAT GGA GTG TAT GGA TC-3' (1261-1284)
  D: 5'-TCC ATT CCG CTC TCC TGA GCA C-3' (1624-1646)
• Opsin: PCR product 217 bp
  U: 5-TGC CTC ACA CAATTC CCA TC-3 (1426-1445)
  D: 5- TGG GAG GTG CCT TCA GAC TA-3 (1643-1626)
• Otx2:
  U 5-GGA TGG ATT TGT TGC ACC AGT C-3
  D 5-CAC TCT CCG AGC TCA CTT CTC-3
• Tanabin: PCR product 397 bp
  U: 5-GAA GGC AAA TGG AGA GCA C-3 (5099-5117)
  D: 5-GCA AAG TCT TAG GGG CAT CC-3 (5496- 5477)
• Xbra: PCR product 187 bp
  U: 5- GGA TCG TTA TCA CCT CTG-3 (1703-1720)
  D: 5-GTG TAG TCT GTA GCA GCA-3 (1890-1873)
• XFS319 Follistatin: PCR product 220 bp
  U: 5-CAG TGC AGC GCT GGA AAG-3 (852-869)
  D: 5-TGC GTT GCG GTA ATT CAC-3 (1081-1064)
• XGATA1:
  U: 5-CAG GAG TTC AGT CTG TTT CAG-3
  D: 5-CAG GGT TCT CAC TTG CAT TC-3
• Xhox3:
  U: 5-ATA TGA TGA GCC ACG CAG CAG-3
  D: 5-CAG ATG CTG CAG CTC TTT GGC-3
• Xif 3.
  U 5-ATC CTC CAG GCT ATC CAC CTC C-3
  D 5- TAG CGG ACC TTC TCT ATG AAG C-3
• Xlhbox6: PCR product 217 bp
  U: 5-TAC TTA CGG GCT TGG CTG GA-3 (253-272)
  D: 5-AGC GTG TAA CCA GTT GGC TG-3 (470-449)
• XMRF4:
  U. 5-CTT TTA CCT GGA TGG AG-3
  D: 5-TGG TGG AGC TAA GAC AT-3
• XMyoDa:
  U: 5-AGC TCC AAC TGC TCC GAC GGC ATG AA-3
  D: 5-AGG AGA GAA TCC AGT TGA TGG AAA CA-3 (615-1009)
• XMyoDb:
  U: 5-AAC TGC TCC GAT GGC ATG ATG GAT TA-3
  D: 5-AAT GCT GGG AGA AGG GAT GGT GAT TA-3 (662-925)
• Xnot:
  U: 5-ATA CAT GGT TGG CAC TGA-3
  Xnot D:CTC CTA CAG TTC CAC ATC
• Xtwi:
  U. 5-AGT CCG ATC TCA GTG AAG GGC A-3
  D: 5-TGT GTG TGG CCT GAG CTG TAG-3 (290-684)
• Xwnt8: PCR product 330 bp
  U: 5-AGA TGA CGG CAT TCC AGA-3 (1127-1144)
  D. 5-TCT CCC GAT ATC TCA GGA-3 (1457-1440)
  -note recent correction to D rpimer 3' end by Fritz Aberger!
• xWT1 (Wilms' tumor) : PCR product 436 bp. Distinguishes -KTS and +KTS forms
  5' CACACSCACGGGGTCT 3' and
  5' TGCATGTTGTGATGRCG 3'
• Xenopus Zic3, (232 bp)
  5'-TTCTCAGGATCTGAACACAT-3'(1651-1670)
  5'-CCCTATAAGACAAGGAATAC-3'(1901-1882)