Methods and materials: antisense training facility
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For those people who are
interested in loss of function studies on maternal Xenopus genes but who need
advice and help on antisense and host-transfer techniques to target their gene
of interest, there is now a small NIH-funded teaching facility available. From
July 1999, the Heasman/Wylie laboratory at the Cincinnati Children's Hospital
Medical Center will be able to offer individual hands-on instruction of the
antisense depletion and host-transfer techniques. Teaching assistance will range
from advice and encouragement, to week-long visits to the core facility to learn
and carry out the antisense depletion and host-transfer techniques. Minorities
and females are encouraged to apply. Financial assistance for travel and lab
expenses will be available from a small fund for needy cases. Contact Janet.Heasman@chmcc.org
for further information.
Antisense News
Those interested in studying
the function of maternal or early zygotic genes may like to try a different antisense
approach. Morpholino oligos (marketed by Gene Tools) are less toxic than phosphorothioates,
and can be injected into fertilized eggs. In recent experiments targetting maternal
beta catenin, a morpholino caused a ventralized phenotype when injected at the
two cell stage. It also continued to affect beta catenin protein levels through
the neurula stage. For more information contact:
Janet.Heasman@chmcc.org
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