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Xine Volume 12 - Issue 1, March 2012

Xenoups Special Issue of Genetics, Genomics and Cell Biology available

The *Xenopus Special Issue on Genetics, Genomics and Cell Biology* is now
published as the March issue of *genesis, The Journal of Genetics and
Development*. All articles are freely available for downloading. Please
go to: to
check out the exciting new information. If you would like a full-sized
poster of the cover art, please send me an email and include your full
mailing address (one per lab please).
Sally A. Moody, Ph.D.
Professor of Anatomy and Regenerative Biology
George Washington University
School of Medicine and Health Sciences
Editor-in-Chief, *genesis, The Journal of Genetics and Development*

Welcome to the Xenopus listserve

Welcome to the new Xenopus mailing list. With the establishment of the National Xenopus Resource (NXR) here at the Marine Biological Laboratory we have decided to centralize certain aspects of how the community relays information. Therefore, Xine has now been incorporated into the NXR and we have established a new listserve for the community to post information. This listserve will be used to announce workshops, courses, frogs for sale, and other issues directly relevant to the community.

We here at the NXR look forward to developing a good relationship with all Xenopus researchers and providing the necessary services required for everyone to benefit.

You should have received an introductory email allowing you to unsubscribe if you would choose to not be part of this listserve. If you know of people who would like to be part of this listserve, please have them sign up at

Should you have any questions, concerns or comments please feel free to email us directly at xenopus at


The National Xenopus Resource Team

P.S. A second email will shortly follow with information regarding the upcoming Xenopus Bioinformatics Workshop being held here at the MBL May 14-20

Marko Horb, Ph.D.
Director, National Xenopus Resource (NXR)
Marine Biological Laboratory
7 MBL Street
Woods Hole, MA 02543

Email: xenopus at
Office: 508-289-7627
Lab: 508-289-7370
Cell: 508-564-3764

Upcoming Xenopus Bioinformatics Workshop

The NXR would like to announce the upcoming Xenopus Bioinformatics Workshop to be held May 14-20 here at the MBL. This workshop is specifically geared towards Xenopus and will be taught by Xenopus researchers. The instructors are Bob Freeman, Leon Peshkin and Mike Gilchrist.

This course is designed to provide hands on training and to teach you:
-work with large datasets specific to Xenopus
-basic coding for data analysis in UNIX
-visualize data on genome browsers
-analyze Xenopus genomics
-analyze transcriptomics
-analyze proteomics data

For more information and to register go to the following link:

Registration is now open and we encourage people to sign up as soon as possible as participation is limited to 25 individuals.


National Xenopus Resource

Marko Horb, Ph.D.
Director, National Xenopus Resource (NXR)
Marine Biological Laboratory
7 MBL Street
Woods Hole, MA 02543

Email: xenopus at
Office: 508-289-7627
Lab: 508-289-7370
Cell: 508-564-3764

I-Sce1 transgenesis plasmids

We want to try using the I-Sce1 protocol for making transgenic laevis
but have some questions about plasmids design.

Some labs use vectors with one I-Sce1 site and others use vectors with 2
inverted I-Sce1 sites. Is one better than the other? I am confused
about how the inverted site vectors would work. Wouldn't the genomic
DNA have to be cut in 2 places for this to work since the sticky ends of
the insert are not complementary? Also if you have the plasmid cut in 2
pieces don't you run the risk of having the vector backbone becoming
integrated into the genome?

Is it advisable to use insulators on either side of the
promoter-gene-SV40 cassette?

Thank you

Jill Johnston

Xenbase: The Xenopus laevis and X. tropicalis resource.
Version: 4.11.3

Major funding for Xenbase is provided by grant P41 HD064556