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XB-ANTIBODY-14579333
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Antibody Name:
Isl1/2 Ab1
Common Name: Islet1 and Islet2 RRID: AB_2314683 Synonyms: 39.4D5, ISL LIM homeobox 1
Source: Developmental Studies Hybridoma Bank |
1/3 
| Antibody | |
| Clone Number | 39.4D5 |
| Purification | |
| Isotype | IgG2b |
| Host Organism | mouse |
| Xenopus Reactivity | X. tropicalis |
| Non-Xenopus Reactivity | chicken, human, mouse, rat, zebrafish |
| Description: | supplied by DSHB, Cat# 39.4D5 Generated by Jessell, T.M. / Brenner-Morton, S. may recognize both Islet1 and Islet2 |
| Immunogen | |
| Name | Islet-1 & Islet-2 homeobox |
| Type | polypeptide |
| Post Translational Modifications | None |
| Source Organism | Other |
| Description | c-terminus of Rat Islet1 and Islet2 from bacterial fusion protein, Recombinant fusion protein containing a.a. 178-349 of Isl1 protein (C-terminus), Predicted: 39kDa; Apparent: 39kDa, This antibody also detects Islet-2 (Uniprot P50480. and Gene ID 57233). The protein region used for immunization lacks the LIM motifs shared with other proteins. |
| Sequence | |
| Reported Usage | |
| immunohistochemistry | MN, DRG & other nuclei characterized extensively by immunocytochemistry; works in paraformaldehyde-fixed and paraffin-embedded tissue; does not work in methanol or acetone (see Sanders et al., 2003, and Georgia et al., 2006) |
| immunofluorescence | 1:100 |
| immunoprecipitation | |
| electrophoretic mobility shift assay | |
| western blot | |
| Publications | |
| ???displayAntibody.papersFirst??? |
Metalloproteases and guidance of retinal axons in the developing visual system., J Neurosci 2002  |
| ???displayAntibody.papersRecent??? |
Zic5 stabilizes Gli3 via a non-transcriptional mechanism during retinal development., Cell Rep 2022  |
| ???displayAntibody.papersAll??? | |
