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Antibody Name: Pum1 Ab3 Common Name: Pumilio RRID: Synonyms: Antigens Gene: pum1 XAO: oocyte Clone Type: unknown Source:

Antibody
Clone Number	Pum2A5
Purification
Isotype	IgG1
Host Organism	mouse
Xenopus Reactivity	X. tropicalis, X. laevis
Non-Xenopus Reactivity
Description:	Generated by Yamashita Lab
Immunogen
Name	GST-XPum1–4R
Type	polypeptide
Post Translational Modifications	None
Source Organism	frog
Description	The recombinant protein GST-XPum1–4R was injected into mice to produce monoclonal antibodies according to the procedures described previously (53). To obtain antibodies specific to XPum1–4R, hybridoma clones were screened with XPum1–4R cleaved from GST with thrombin (50). One clone (Pum2A5, IgG1 with a κ light chain) was selected to detect XPum by immunoprecipitation and immunoblotting.
Sequence
Reported Usage
immunoprecipitation
western blot
Publications
???displayAntibody.papersFirst???	Biochemical identification of Xenopus Pumilio as a sequence-specific cyclin B1 mRNA-binding protein that physically interacts with a Nanos homolog, Xcat-2, and a cytoplasmic polyadenylation element-binding protein., J Biol Chem 2001
???displayAntibody.papersRecent???	Involvement of Xenopus Pumilio in the translational regulation that is specific to cyclin B1 mRNA during oocyte maturation., Mech Dev 2003
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