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XB-ANTIBODY-14578966

Antibody Name: Cdh1 Ab1

Common Name: E-cadherin

RRID: AB_528116
Synonyms: 5D3, Cdh1 Antibody (5D3) , XIE-cadherin

Antigens
Gene: cdh1
XAO: ectoderm, epithelium

Clone Type: Monoclonal
Source: Developmental Studies Hybridoma Bank

Antibody
Clone Number 5D3
Purification
Isotype IgG2b
Host Organism mouse
Xenopus Reactivity tropicalis, laevis
Non-Xenopus Reactivity fruitfly
Description: Generated by Barry Gumbiner , University of Virginia. Supplied by DSHB, Cat # 5D3. Citation: (DSHB Cat# 5d3, RRID:AB_528116). Please email citation to DSHB. Alternate Gene Names: arc-1/cd324/cdhe/ecad/lcam/uvo
Immunogen
Name E-cadherin
Type polypeptide
Post Translational Modifications Glycosylation
Source Organism frog
Description Purified E-cadherin extracellular domain (100 kDa trypsin fragment ectodomain) from Xenopus A6 kidney cell line. Blocks epithelial cell-cell adhesion and cell junction formation. Stains ectoderm, all epithelia and regions of cell-cell contact. E-cadherin-specific by immunoblotting and immunoprecipitation.
Sequence
Reported Usage
western blot
immunoprecipitation
immunofluorescence
Publications
First: Expression of cell adhesion molecule E-cadherin in Xenopus embryos begins at gastrulation and predominates in the ectoderm., J Cell Biol 1989  
Most recent: Molecular markers for corneal epithelial cells in larval vs. adult Xenopus frogs., Exp Eye Res 2019                          
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