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Summary Images Attributions Wiki Source
XB-ANTIBODY-14580344

Antibody Name: Trim36 Ab1

Common Name:

RRID:
Synonyms:

Antigens
Gene: trim36

Clone Type: unknown
Source:

Antibody
Clone Number
Purification
Isotype isotype unknown
Host Organism guinea pig
Xenopus Reactivity tropicalis, laevis
Non-Xenopus Reactivity
Description: Created by Cuykendall et al. The trim36 CDS was cloned into the pDEST17 vector (invitrogen) by Gateway recomb to generate a fusion protein N-terminally tagged with 6-His. Recombinant protein was produced in bacteria, and refolded from inclusion bodies as described (Houston and King, 2000a). The 6-His-tagged Trim36 protein was then purified over a nickel column (His GraviTrap, GE Healthcare), eluted with 300mM imidazole and dialyzed against PBS.
Immunogen
Name Trim36
Type polypeptide
Post Translational Modifications None
Source Organism frog
Description
Sequence
Reported Usage
Publications
First: Vegetally localized Xenopus trim36 regulates cortical rotation and dorsal axis formation., Development 2009        
Most recent: Maternal Dead-End1 is required for vegetal cortical microtubule assembly during Xenopus axis specification., Development 2013                            
View All Papers

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