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XB-ANTIBODY-14586631

Antibody Name: Pum1 Ab3

Common Name: Pumilio

RRID:
Synonyms:

Antigens
Gene: pum1
XAO: oocyte

Clone Type: unknown
Source:

Antibody
Clone Number Pum2A5
Purification
Isotype IgG1
Host Organism mouse
Xenopus Reactivity tropicalis, laevis
Non-Xenopus Reactivity
Description: Generated by Yamashita Lab
Immunogen
Name GST-XPum1–4R
Type polypeptide
Post Translational Modifications None
Source Organism frog
Description The recombinant protein GST-XPum1–4R was injected into mice to produce monoclonal antibodies according to the procedures described previously (53). To obtain antibodies specific to XPum1–4R, hybridoma clones were screened with XPum1–4R cleaved from GST with thrombin (50). One clone (Pum2A5, IgG1 with a κ light chain) was selected to detect XPum by immunoprecipitation and immunoblotting.
Sequence
Reported Usage
western blot
immunoprecipitation
Publications
First: Biochemical identification of Xenopus Pumilio as a sequence-specific cyclin B1 mRNA-binding protein that physically interacts with a Nanos homolog, Xcat-2, and a cytoplasmic polyadenylation element-binding protein., J Biol Chem 2001  
Most recent: Involvement of Xenopus Pumilio in the translational regulation that is specific to cyclin B1 mRNA during oocyte maturation., Mech Dev 2003  
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