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XB-ANTIBODY-16325890
Antibody Name:
hpse2 Ab1
Common Name: heparanase 2 RRID: Synonyms:
Source: |
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Antibody | |
Clone Number | |
Purification | |
Isotype | isotype unknown |
Host Organism | rabbit |
Xenopus Reactivity | X. tropicalis |
Non-Xenopus Reactivity | |
Description: | |
Immunogen | |
Name | |
Type | polypeptide |
Post Translational Modifications | None |
Source Organism | frog |
Description | A custom rabbit anti-heparanase 2 antibody was designed and manufactured (Generon). The immunising peptide sequence used was QLDPSIIHDGWLDC (Figure 1) |
Sequence | [+] |
Reported Usage | |
western blot | To test specificity, anti-heparanase 2 antibody was incubated overnight at 4°C on a rotator with a 50 fold excess immunising peptide or a control reaction of 50 fold excess non-specific peptide, prior to use in immunohistochemistry applications. Western blotting was performed by standard methods. 22  Downloaded from http://hmg.oxfordjournals.org/ at Cincinnati Children's Hospital Library on April 8, 2014 Total protein was extracted from pools of three embryos, with amounts equivalent to one embryo separated via SDS-PAGE. |
Publications | |
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Heparanase 2, mutated in urofacial syndrome, mediates peripheral neural development in Xenopus., Hum Mol Genet 2014 |
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